Review Article - Journal of Food Nutrition and Health (2022) Volume 5, Issue 6
Presence of Pesticide Residue in Fruits and Vegetables and Their Effect on Human Health: A ReviewDahikar SB1*, Bhutada SA1, Girase MS2
- *Corresponding Author:
- Dahikar SB
Department of Microbiology
Savitribai Phule Pune University
E-mail: [email protected]
Received: 10-Oct-2022, Manuscript No. AAJFNH-22- 76851; Editor assigned: 12- Oct -2022, PreQC No. AAJFNH -22-76851 (PQ); Reviewed: 27- Oct-2022, QC No. AAJFNH -22- 76851; Revised: 01-Nov-2022, Manuscript No. AAJFNH -22-76851(R); Published: 08-Nov-2022, DOI: 10.35841/aajfnh-5.6.126
Citation: Dahikar SB, Bhutada SA, Girase MS. Presence of pesticide residue in fruits and vegetables and their effect on human health: A review. J Food Nutr Health.2022;5(6):126
Pesticides, Neurodegenerative disorders, Cancer, Oxidative stress, Infertility.
Pesticides are commonly used to remove pests from the standing crops, if not taken care of these pests can severely affect the crops and reduce agricultural output. The concept of pesticides has been in existence since 2000 BC. In the Sumer civilization of ancient Mesopotamia around 4,500 years ago sulfur dust was used as a pesticide. Rigveda also mentions the use of plants that were toxic to pests . Pests like weeds, plant diseases and insects are a challenge to farmers. Every year about 45% of production is lost due to pest infestation. In order to reduce these losses, different types of pesticides are used (Table 1). Annually about 2 million tons of pesticides are used around the globe. China stands 1st followed by the U.S.A, Argentina and India occupy 12th position in pesticide use . Paddy and cotton occupy a major portion of pesticide use i.e. 18 and 50% of the total, though they cover only a small portion of the cropped area .
|Type of Pesticide||Role|
|Fungicides||Inhibits growth of fungal strains; reduces blights, rusts, mildews & molds in plants. Ex. Pentachlorophenol (Ito et al., 2022)|
|Herbicides||Controls growth of nuisance plants that grow in close vicinity with crops. Ex. Glyphosate|
|Fumigants||Controls the growth of pests residing in soil by forming a gas that can disturb their growth. Ex. Dichloropropane|
|Insecticides||Inhibits the growth of insects that damage the crops. Ex. Lindane|
|Microbial pesticides||Microbes They are highly specific microbes that can inhibit growth or can potentially kill pests. Ex. Bacillus thuringienesis|
|Miticides||Also known as acaricides, they control ticks & mites’ infestations. Ex. Azobenzene|
|Molluscicides||Control gastropod infestation in crops by killing slugs & snails.
|Ovicides||Controls infestation by mites, nematodes, or insects by destroying their eggs. Ex. Pyriproxyfen|
|Nematicides||Protects crops from nematodes (threadworms, roundworms).
|Pheromones||Control the population of pests by disrupting the sexual cycle of pests.|
|Repellants||Repels pests like insects, and birds from crops.
|Defoliants||Causes abscission (early shedding of leaves).
Ex. 2,4-Dichlorophenoxyacetic acid
|Rodenticides||Protects crops from rodents (rats, squirrels, chipmunks, etc.)
Ex. Cholecalciferol, Zinc phosphide
|Desiccants||Causes necrosis, leaf shedding in weeds.
|Insect growth regulators||Disrupt the various stages of the life cycle of any pests from molting to the adult stage. Ex. Halofenozide|
Table 1. Different types of pesticides on the basis of their functions (Maurya & Malik, 2016).
Commonly used pesticides
Monocrotophos, Acephate, Endosulfan, Carbofuran, Chlorpyrifos, Lindane, etc. are some of the commonly used pesticides around the globe. There are several criteria for the classification of pesticides ranging from the target organism & to the type of crop [4,5].
On the basis of chemical nature, pesticides can be classified as:
Organochlorines: consists of chlorinated compounds. They are persistent and accumulate in adipose tissues. e.g., Hexachlorobenzene .
Carbamic derivatives: derivatives of Dimethyl N-Methyl Carbamic acid. They have short half-life. e.g., Mollinate .
Organophosphates: derivatives of phosphoric acid and they have direct effects on CNS. e.g., Malathion .
Fate of pesticides in soil
When a pesticide is applied to crops it is affected by several processes including breakdown, adsorption, degradation and transfer. Transfer processes include adsorption, spray drift, leaching, volatilization and runoff . Through adsorption, the pesticide binds to soil particles and this depends on the type of soil, type of pesticide, moisture content and pH of soil. The pesticide that is adsorbed is neither leached nor taken up by plants . Through volatilization, the solid or liquid pesticides change their form and get converted to gaseous form as a result of it they move away from the site of application resulting in vapor drift. This can damage the nearby crops . When the dust or droplets of pesticide move through the air away from the site of application it is referred to as Spray Drift. This drift can damage or contaminate nearby crops and can severely affect the environment . When pesticide moves over a slope along with the water it is referred to as runoff. It results in the contamination of ponds, streams, lakes & rivers . When water moves through soil and contaminates the groundwater it is known as leaching. It mostly occurs in the case of water-soluble pesticides and sandy soil . Sometimes plants uptake the pesticides present in the soil, they either get degraded but there are some pesticides that are resistant to degradation; consumption of these plants in any form causes their accumulation in living tissues resulting in toxicity .
Effects of pesticide residue on human health
There has been a constant rise in the detrimental effects of pesticides on human health. Pesticides enter our body via food i.e. fruits or vegetables, polluted water, direct contact, and the after-effects can be acute as well as chronic . Acute illness develops shortly after the contact and is characterized by dizziness, nausea, tremors, headaches, panic attacks, etc. Continued exposure often results in chronic illness characterized by hormonal imbalances, respiratory diseases, neurodegenerative disorders, cancer, birth defects, diabetes, reproductive disorders, and renal disorders .
Pesticides enter the human body via dermal, oral, or inhalation exposure. Dermal exposure mostly occurs during the handling and mixing of pesticides. Exposure via inhalation occurs when pesticides are in form of dust, vapor, or fine powder. Different pesticides and their metabolites affect different body parts, for example, DDT affects sexual development in humans, and chlorpyrifos affects the nervous system .
Neuronal Damage, Neuropsychiatric Disorders & Cognitive Defects
Organophosphates and carbamates result in acute toxicity; they hydrolyze acetylcholine which is a major neurotransmitter for CNS & PNS, hydrolysis results in AChE buildup at cholinergic synapses, which results in overstimulation of receptors . Cholinergic receptors are distributed across all major organs of the body their overstimulation increases bronchoconstriction, GI motility, tremors, diarrhea, sweating, bronchial secretion, salivation, etc. It inhibits the respiratory centers and causes paralysis of respiratory muscles resulting in respiratory depression. Metabolites from these pesticides stimulate glutamatergic neurons resulting in over secretion of glutamate causing seizures, irreversible brain damage, and behavioral disorders . Neural cell death results in neuropsychiatric impairments leading to loss of memory & concentration, sensory & motor defects, behavioral problems & loss of speech. Recovery from acute illness is possible but brain damage is irreversible. Damage to cholinergic neurons present in the basal forebrain severely affects cognitive ability .
Another disorder associated with organophosphate exposure is OPIDP (Organophosphate Induced Delayed Polyneuropathy). It results in loss of senses, muscle weakness, and tingling of hands. These symptoms are visible after a single exposure .
Developmental neurotoxicity & CNS disorders are other long-term effects of organophosphates. These OPs can disturb major cellular processes in humans (Neurite growth & DNA replication) . DDT is a major pesticide discovered in 1939, through oral route it has moderate toxicity, acute exposure results in increased spontaneous movement, coarse tumors, respiratory failures, motor unrest) .
Free radicals are chemical species with an unpaired electron in their outermost shell, these unpaired electrons are highly reactive, and they easily accept or donate an unpaired electron to become a stable molecule. Oxygen is required for almost every activity that our body performs like oxidation, respiration, and energy generation. Under normal circumstances, O2 can accept 4 electrons resulting in the formation of two water molecules. Under special circumstances, this balance is disturbed leading to the formation of ROS . ROS is capable of causing oxidative damage to biomolecules like lipids, and proteins. Lipids present in cell membranes are more susceptible to this kind of attack as they are rich in methylene groups. All these processes proceed to peroxidative damage . Counteractive mechanisms are present in the human body for neutralization of damage but an imbalance in these mechanisms results in lipid peroxidation. This increases the fragility of erythrocytes and alters the fluidity of the erythrocyte membrane . Organophosphate pesticides can alter glucose homeostasis resulting in the development of insulin resistance, diabetes, pancreatitis, and DNA damage. Thus, oxidative stress can play an active role in the progression of multiple diseases .
Chronic exposure to pesticides results in respiratory, immune, nervous & reproductive disorders (Table 2). They can cause direct damage to the reproductive system or disturbs the endocrine balance resulting in indirect damage. Pesticides are also known as endocrine disrupters as they severely affect the hormonal pathway by affecting the release of Gonadotropin- Releasing Hormone, Follicle Stimulating Hormone & Luteinising Hormone . In males, the testis is solely responsible for the synthesis of steroid hormones and the formation of sperms. Different pesticides have different effects on the male reproductive system. Ref in table . In females, pesticides disturb ovarian physiology and alter the secretion of hormones. Alteration in the hormonal system disturbs the maturation of follicles, the ovarian cycle, stillbirth, infertility, and prolonged pregnancy .
|Pesticide||Effect on Male Reproductive System|
|Aldrin||Binds to receptors for Androgen|
|Lindane||Luteal progesterone decrease binds to androgen, estrogen, and progesterone receptors|
|Fenoxycarb||Disturbs pathway of testosterone|
|Parathion||Inhibits synthesis of gonadotropin hormone|
|Chlordane||Binds to receptors for androgens|
Table 2. Effect of Pesticides on Male Reproductive System (Ngolula et al., 2012).
For the development and progression of cancer occupational exposure is a major trigger. Exposure occurs mainly via dermal contact, inhalation, or ingestion . Exposure to high concentrations of pesticides within a short span results in acute disorders, the chronic effects of exposure are observed after a few months or even years. Occupational exposure is also a major concern and major risk groups include workers of the production unit, transporters, distributors, and field workers .
Free radicals arising from oxidative damage cause damage to biomolecules, depletes the concentration of antioxidants present in the cell, damages DNA, induces mutations, and cause breaks in DNA & chromosomes. This xenobiotic also affects the functioning of glands like the thyroid, pituitary & hypothalamus glands, and affects the synthesis and action of hormones . These pesticides also cause immunosuppression, all of these effects result in the development and progression of cancer .
Pesticides can lead to the development of about 45 types of cancer out of which bladder cancer, prostate cancer, myeloma & non-Hodgkin lymphoma are the most common. Multiple myeloma is hematopoietic cancer and is most commonly reported in farm and factory workers .
Carcinogens can be divided into 4 categories:
Group A: Relationship between cancer development and exposure to these carcinogens is backed by several epidemiological studies. Most the pesticides are excluded from this category except Ethylene oxide which is used as a fumigant .
Group B: Relationship between cancer development and exposure to these carcinogens is backed by animal models but there is a lack of epidemiological studies. About 28 pesticides belong to this category including Aldrin, DDT, Telone, Fenoxycarb, Lindane, Flopet, Lactofen, PCP, etc. .
Group C: Relationship between cancer development and exposure to these carcinogens is fewer studies as animal studies provide limited data and there is no data related to epidemiological studies. About 15 pesticides belong to this category including Atrazine, Bromacil, Diclofol, Fipronil, Isoxaben, Moline, Parathion, Phosmet, etc. .
Group D: There is no data related to this group and no pesticide falls under this category.
Ways to reduce pesticide toxicity
Excess use of pesticides not only affects the quality of soil over time but also affects the health of the individuals involved. Different countries use different kinds of pesticides which have varied effects on the human system. Due to a lack of suitable alternatives stopping the use of these pesticides is not possible but certain strategies can be adopted for reducing their use as well as the toxicity caused by them .
It involves prohibiting the use of all kinds of synthetic chemicals for increasing productivity. These practices rather depend on biological and mechanical ways. At present, about 24 million hectares of land are used for organic farming around the world. Organic farming is based on 4 principles namely: Principles of health, ecology, fairness, and care. The output of organic farming is more nutritional, free from toxins, energy, cost-efficient, environmentally friendly, and improves the quality of the soil. Some crops can even aid in carbon sequestration. It is a step towards long-term sustainability that will not only protect us from the toxic effects of pesticides but will save the environment from further degradation. But like every other method it also has some limitations like it is time-consuming, productivity is low and the requirement for skilled labor [41,42].
Processing Food Products
Processing of agricultural produce includes washing, peeling, grinding, boiling, fermentation, drying, and canning. Pesticide residues that are present on the surface can be removed with washing which is the most common practice adopted in households for cleaning agricultural produce. Several sanitizing solutions are also available on market for cleaning. The efficiency of washing is dependent on several factors like the type of solution used for washing, type of pesticide, contact time with pesticide & nature of the agricultural product . The majority of pesticides are not able to penetrate through the skin of fruits and vegetables, peeling them are also a suitable option. About 70% of the residues can be removed simply by peeling .
Cooking also reduces the concentration of pesticides present in food. Processing of food via boiling can remove about 35- 60% of OP residues (Organophosphate) .
Rational use of Pesticides
Chemical fertilizers and pesticides not only affect human health but also have adverse effects on soil fertility and the environment. They cause bioaccumulation in humans and other animals, reduce fertility over time, contaminate groundwater and cause degradation of soil. To reduce the ill effects, their rational use is recommended, this approach involves the selection of appropriate pesticides, their proper dilution, management of dosage rates, application frequency, and method of application. All these approaches can effectively limit residues in agricultural products. In some cases, they can also be replaced with biopesticides .
Use of Biopesticides
Out of total plant protectants used around the globe biopesticides’ share is only 2 %. But in recent years the global production has reached over 3,000 tons annually. The use of biopesticides is advancing at a rate of 10% per year . Biopesticides are of three types namely microbial, biochemical and plant-incorporated pesticides. Microbial pesticides are highly selective and include bio fungicides (Pseudomonas), bioherbicides, and bioinsecticides. Among all microbial pesticides Bacillus, thermogenesis is commonly and accounts for 90% of all biopesticides currently used . Herbal extracts can also be used for the management of pests. These alternatives not only reduce pest infestation in crops but also prevents bioaccumulation of chemicals in agricultural products .
Though pesticides have an important role to play in agriculture but the harm it poses cannot be neglected. They pose acute risks as well as chronic effects ranging from neurodegenerative disorders, oxidative stress, infertility in both males and females, tumors, and cancer of all types. But to meet the growing demands and to feed the growing population high agriculture output is desired for which we are dependent on pesticides. Since there is no suitable alternative present so complete boycott is also not possible. But combining all alternatives may yield fruitful results. Reducing the use of pesticides and replacing them with biopesticides, adapting to sustainable agricultural practices and organic farming, and proper washing and processing of food before consumption can help to reduce damage. More focus is required on the development of biopesticides so that dependency on chemicalbased pesticides can be reduced.
- Manyilizu WB. Pesticides, anthropogenic activities, history and the health of our environment: lessons from Africa. In Pestici-Use and Misuse Their Imp Envir. 2019;111-20.
- Sharma A, Kumar V, Shahzad B, et al. Worldwide pesticide usage and its impacts on the ecosystem. SN Appl Sci. 2019;1(11):1-16.
- Matthews G. Pesticides: health, safety and the environment. John Wiley & Sons. 2015.
- Jyothi I. Devi S. A- Review on pesticide residues of fruits and vegetables. Inter J Res in Appli Nat & Soci Sci. 2016;4(11):71-8.
- Maurya P, Malik A. Bioaccumulation of xenobiotics compound of pesticide in riverine system and its control technique-A critical review. J Indust Pollu Contr. 2016;32(2):580-94.
- Ensley SM. Organochlorines Veterinary Toxicology. Acade Press. 2018;509-13.
- Reigart JR, Roberts JR, Recognition and Management of Pesticide Poisonings. Uni Sta Enviro Prote Age.735K-13001.2013.
- El Nemr A, El‐Said GF, Khaled A. Risk assessment of Organochlorines in mollusk from the Mediterranean and Red Sea coasts of Egypt. Water Enviro Res. 2016;88(4):325-37.
- Mottes C, Lesueur-Jannoyer M, Le Bail M, et al. Pesticide transfer models in crop and watershed systems: a review. Agron Sustain Dev. 2014;34(1):229-50.
- Chang CF, Lee SC, Adsorption behavior of pesticide methomyl on activated carbon in a high gravity rotating packed bed reactor. Water Resear. 2012;46(9):2869-80.
- Garcia L, Bedos C, Génermont S, et al. Modeling pesticide volatilization: testing the additional effect of gaseous adsorption on soil solid surfaces. Environ Sci Tech. 2014;48(9):4991-98.
- Bueno MR, da Cunha JPA, de Santana DG. Assessment of spray drift from pesticide applications in soybean crops. Biosyst Engine.2017;154:35-45.
- Chen C, Guo W, Ngo HH, Pesticides in storm water runoff—A mini review. Front Env Sci Engin.2019;13(5):1-12.
- Pérez-Lucas G, Vela N, El Aatik, et al. Environmental risk of groundwater pollution by pesticide leaching through the soil profile. Pesticid-use and misuse their imp enviro. 2019;1-28.
- Maurya P, Malik A. Bioaccumulation of xenobiotics compound of pesticide in riverine system and its control technique-A critical review. J Indust Pollu Contr. 2016;32(2):580-94.
- Blair A, Ritz B, Wesseling C, et al. Pesticides and human health. Occup Env Medic. 2015;72(2):81-2.
- Sharma N, Singhvi R. Effects of chemical fertilizers and pesticides on human health and environment: a review. Int J Agric Envir Biote. 2017;10(6):675-80.
- Hallenbeck WH, Cunningham-Burns KM. Pesticides and human health. Spring Sci Busi Media. 2012.
- Jokanovic M. Neurotoxic effects of organophosphorus pesticides and possible association with neurodegenerative diseases in man: A review. Toxicol. 2018;410(1):125-31.
- Gunnarsson L, Bodin L, Occupational Exposures and Neurodegenerative Diseases—A Systematic Literature Review and Meta-Analyses. Environ Rese and Pub Heal. 2018;16(3):337.
- Arab A, Mostafalou S. Neurotoxicity of pesticides in the context of CNS chronic diseases. Int J Envi Hea Res. 2021.
- Jokanovic M. Neurotoxic effects of organophosphorus pesticides and possible association with neurodegenerative diseases in man: A review. Toxico. 2018;410(1):125-31.
- Theodore A, Slotkin, Frederic J Seidler. Developmental neurotoxicity of organophosphates targets cell cycle and apoptosis, revealed by transcriptional profiles in vivo and in vitro. Neuroto Terato. 2012. 34(2):232-41,
- Kajta M, Wójtowicz AK. Impact of endocrine-disrupting chemicals on neural development and the onset of neurological disorders. Pharma Rep. 2013;65:1632–39.
- Husak VV, Copper and copper-containing pesticides: metabolism, toxicity and oxidative stress. J Vasyl Stefanyk Precarp Natio Uni. 2015;2(1):39-51.
- Yang C, Lim W, Song G. et al. Mediation of oxidative stress toxicity induced by pyrethroid pesticides in fish.Comp Biochem and Phy Part C: Toxi & Pharma. 2020;234-58.
- de Oliveira JSP, Vieira LG, Carvalho WF, et al. Mutagenic, genotoxic and morphotoxic potential of different pesticides in the erythrocytes of Podocnemis expansa neonates. Sci Total Envir. 2020; 737:140304.
- Mostafalou S, Abdollahi M. Pesticides and human chronic diseases: evidences, mechanisms, and perspectives. Toxico Appli Phar. 2013;268(2):157-77.
- Sweeney T, Nicol L, Roche JF, et al. Maternal exposure to octylphenol suppresses ovine fetal follicle stimulating hormone secretion, testis size, and Sertoli cell number. Endocrinology. 2000;141:2667-73.
- Ngoula F, Ngouateu OB, Kana JR, et al. Reproductive and developmental toxicity of insecticides. Adva in Integ Pest Mana. 2012;429-57.
- Sharma RK, Singh P, Setia A, et al. Insecticides and ovarian functions. Enviro Molecu Mutagen. 2020; 61:369-92.
- Cuenca JB, Tirado N, Vikström M, et al. Pesticide exposure among Bolivian farmers: associations between worker protection and exposure biomarkers. J Expo Sci Environ Epidem. 2019;30:730–42.
- Kalliora C, Mamoulakis C, Vasilopoulos E, et al. Association of pesticide exposure with human congenital abnormalities. Toxicolo Appli Pharm. 2018;346:58–75.
- Rashidi M, Mahabadi H, Khavanin A. Evaluation of the efects of chronic exposure to organophosphorus pesticides on thyroid function. Asia Pacifc J Med Tox. 2020;9:35–43.
- Mostafalou S, Abdollahi M. Pesticides: an update of human exposure and toxicity. Arch Toxicol. 2017; 91:549–99.
- Ferri GM, Specchia G, Mazza P, et al. Risk of lymphoma subtypes by occupational exposure in Southern Italy. J Occup Med Toxicol. 2017;12:31.
- Ali G. Energy-aided environmental sustainability assessment of an ethylene dichloride-vinyl chloride production process. Chem Engin Res Desi. 2018;109-28.
- Clausing P, Robinson C, Schaden H, et al Pesticides and Public Health: an analysis of the regulatory approach to assessing the carcinogenicity of glyphosate in European Union. J Epi Com Hea. 2018.72(8):668-72.
- Varghese J, Sebastian E, Iqbal T, et al. Pesticide applicators and cancer: a systematic review. Reviews on environmental health. 2021;36(4):467-76.
- Gale F, Buzby JC. Imports from China and food safety issues. Eco Inf Bul. 2009;52:1-37.
- Niggli U, Slabe A, Schmid O, et al. Vision for an organic food and farming research agenda to 2025. Orga kno for the fut. 2008.
- Butler G, Nielsen JH, Slots T, et al. Fatty acid and fat-soluble antioxidant concentrations in milk from high- and low-input conventional and organic systems: seasonal variation. J Sci Food Agric. 2008.88: 1431–41.
- Al-Taher F, Chen Y, Cappozzo J. et.al. Reduction of pesticide residues in tomatoes and other produce. J Food Prot. 2013;76(3):510-15.
- Boulaid M, Aguilera A, Camacho F, et al. Effect of household processing and unit-to-unit variability of pyrifenox, pyridaben and tralomethrin residues in tomatoes. J Agri Food Chem. 2005;53:4054–58.
- Holland PT, Hamilton D, Ohlin B, et al. Pesticides report 31: effects of storage and processing on pesticide residues in plant products (technical report). Pure Appl Chem 1994;66(2):335-46.
- Swapna Latha Aggani.2013. Development of biofertilizers and its Future Perspective. J Phar.2013; 2(4):327‐32.
- Bailey KL, Boyetchko SM, Längle T. Social and economic drivers shaping the future of biological control: a Canadian perspective on the factors affecting the development and use of microbial biopesticides. Biol Cont. 2010;52:221-29
- Hubbard M, Hynes RK, Erlandson M, et al. The biochemistry behind biopesticide efficacy. Sustain Chem Proc. 2014;2:18.
- Gupta S, Dikshit AK, Biopesticides: An eco-friendly approach for pest control. J Biopest 2010;3(1): 186-88.