Research Paper - Biomedical Research (2020) Volume 31, Issue 2
Transient replication of human papillomavirus type 16 in HEK293T cells.
Our knowledge regarding human papillomaviruses (HPVs) replication remains very limited, in part due to the lack of a cell culture system for HPV replication. Here we present evidence that the plasmid containing the full-length HPV-16 genome can replicate and produce virus-like particles (VLPs) in HEK293T cells. At 48 h after transfection of the plasmid containing HPV-16 genome into HEK293T cell line, HPV-16 VLPs were observed from the cells with an electron micrograph, the virus DNAs existed in HEK293 cells for at least 60 h, and the fresh HPV-16 DNAs were synthesized at 20 h after transfection. The transcripts of E1, L1 and E1^E4 also were detected by RT-PCR. Therefore, HPV-16 genes expressed and HPV-16 VLPs formed in the cells. These results suggest that HEK293T cells allow HPV-16 replicates transiently.Author(s): Amutikari Mamuti, Qian Shi, Tao Lv, Zhenghai Ma