Biomedical Research

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.
Reach Us +1-504-608-2390

Research Article - Biomedical Research (2018) Volume 29, Issue 21

The positive effect of black cumin ethanol extract (Nigella sativa) on decreasing serum TNF-α levels and interleukin-8 levels in mice model of preeclampsia

Background: During placental preeclampsia experiencing oxidative stress, oxidative stress can increase the expression and activation of nuclear factor-kappa B which regulates the levels of TNF α (Tumor necrosis factor-α), interleukin-6, interleukin-8, COX-2 (Cyclooxygenase-2) genes.

Objective: Analyzing the positive effects of black cumin ethanol extract (Nigella sativa) on reducing serum TNF-α levels and serum interleukin-8 in mice model preeclampsia.

Methods: Research design True experimental with type post-test only control group design. Research using pregnant Balb/c strain mice was healthy used as preeclampsia model mice and divided into 6 groups. Ethanol extract of seeds is Nigella sativa gave orally in the treatment group from 15 to 19 d at a dose of 500 mg kg/d, 1000 mg/kg/d. 1500 mg/kg/d, 2000 mg/kg body weight/d, and terminated at 20th gestational age. Measurement of TNF-α levels and Interleukin-8 levels using ELISA (Enzyme-linked immune sorbent assay).

Results: TNF-α levels and interleukin-8 levels showed a decreased level after being given treatment at a dose of 1500 mg/kg body weight.

Conclusions: Black cumin extract has a positive effect to reduce serum TNF-α and serum interleukin-8 levels to be used as a therapy for preeclampsia disorders.

Author(s): Hamimatus Zainiyah, Ni Wayan Noviani, Rubiati Hipni

Abstract Full Text PDF

Get the App

best replica reviews