Journal of Plant Biotechnology and Microbiology

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Short Article - Journal of Plant Biotechnology and Microbiology (2018) Volume 1, Issue 2

The International Debate on Cross resistance is the cause for multi-drug resistance among soil flora

 Multi-drugs resistance among pathogenic bacteria is menaced in the recent past. Bacteria exposed to xenobiotics at sub-lethal concentration develop resistance through nonspecific hydrolysis of antibiotics. Soil isolates Bacillus cereus showed resistance against chloramphenical, monochrotophos, ampicillin, cefotaxime, streptomycin and tetracycline. Multi-drug resistant properties of this particular strain have been confined to the plasmid, which was verified using plasmid curing by exposing to 2% sodium dodecyl sulfate and were able to resume multi-drug resistance properties once the plasmid was transformed back to bacteria. Further the plasmid DNA was sequenced on MiSeq using 2x300 bp chemistry to generate approximately 1 GB of data. The Draft assemblies of short Illumina sequence reads (2x300 Mi-Seq library) was analyzed by 4200 tape station system. The presence of hydrolases and hypothetical proteins suggest that the plasmid is capable of degrading antibiotics and thus responsible for multi-drug resistance.

Author(s): A Murugan

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