Research Article - Journal of RNA and Genomics (2022) Volume 18, Issue 4

Separation and the study of genetic diversity of a number of strains of Dunaliella microalgae using PCR-RFLP molecular marker Cleaved Amplified Polymorphic Sequence (CAPS)

Today, Dunaliella algae has received a lot of attention due to its high ability to produce beta-carotene, its applications in the food, pharmaceutical, cosmetic and research industries such as genetic engineering. Therefore, further identification of this genus and its productive species will be very useful. Common taxonomy of the Donalilla genus is usually based on its morphological and physiological traits, since these traits can change under different growth conditions due to the lack of a hard cell wall. For this reason, physiological and morphological studies have not been sufficient and have caused confusion in the systematic nature of this genus. Therefore, in this study, molecular markers were used to identify this species better and more accurately. The present study conducted at the northwestern and western agricultural biotechnology research institute in Tabriz which contains a good collection of Dunaliella microalgae. In this experiment, the genetic diversity of a number of Iranian Dunaliella microalgae strains has been investigated using the PCR-RFLP (CAPS) molecular marker. After DNA extraction, the polymerase chain reaction was performed using a pair of protected primers in the ITS fragment, then enzymatic cutting was performed on PCR products of different strains using MseI, Bsp143I and NcoI restricted enzymes. By further examining the pattern of banding of different strains and the value of bands, the diversity of the ITS area of these strains was investigated. The results of the CAPS markers showed that the G45, Q1, Q41 strains and the standard D. salina sample (3.19) had 100% similarity. Given the role of ITS sequence in species identification, this can be said to be categorized in D. salina group.

Author(s): Azam Panahpour, Reza Mohammadi, Nader Chaparzadeh

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