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Research Article - Asian Journal of Biomedical and Pharmaceutical Sciences (2024) Volume 14, Issue 103
RP-HPLC Method for simultaneous Estimation of Metformin and lingaliptin in Pharmaceutical formulation
A simple, Accurate, precise method was developed for the simultaneous estimation of the Linagliptin and Metformin in Tablet dosage form. Chromatogram was run through Ascentis C18 150 x 4.6 mm, 5?m. Mobile phase containing Buffer: Acetonitrile taken in the ratio 65:35 was pumped through column at a flow rate of 0.9 ml/min. Buffer used in this method was 0.1% OPA (2.2ph) buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 216.0nm Retention time of Linagliptin and Metformin were found to be 2.965 min and 2.247 min. %RSD of the Linagliptin and Metformin were and found to be 0.9 and 0.7 respectively. %Recovery was obtained as 100.04% and 99.66% for Linagliptin and Metformin respectively. LOD, LOQ values obtained from regression equations of Linagliptin and Metformin were 0.03, 0.05 and 0.002, 0.005 respectively. Regression equation of Linagliptin is y = 572521x + 1811.1.and y = 132021x + 122105 of Metformin. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.
Author(s): Erri Prashanth Reddy*, Dr. G. Nagamallik