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Research Article - Asian Journal of Biomedical and Pharmaceutical Sciences (2023) Volume 13, Issue 100
Purification and characterization of lectin from Indian borage leaves (Plectranthus amboinicus)..
Lectins are glycan-binding proteins that have been of interest to researchers because of their multi-physiological role. The demonstration of the functionality of lectin from Indian borage leaves (Plectranthus amboinicus) with respect to its antioxidant activity is inadequate. In the present study, lectin was extracted and purified from the leaves of Indian borage by affinity chromatography using Seralose-4B beads that were coupled with dextrose sugar. The purified lectin exhibited haemagglutination activity (HA) with chicken erythrocytes, which showed a higher glycan-specificity to dextrose. Purified Plectranthus amboinicus lectin (PAL) exhibited a molecular weight of 19 kDa and 24 kDa by SDS-PAGE, indicating that this haemagglutinin exists as a dimer composed of two subunits of different molecular weights. The effects of different pH and temperature as well as influence of divalent cations on lectin activity were examined. The PAL activity was constant over a broad range of pH and temperature, and its activity was stable in the presence of divalent cations such as Mg2+ and Ca2+. The basic structure of PAL was studied by FTIR, which revealed that its secondary structure contains α-helix structure. Further examination of PAL bioactivity by the DPPH assay showed an IC50 value of 11.44 µg/mL. These results provide insight into the benefits of Indian borage lectin that can be further explored for its therapeutic value.
Author(s): Roopashri N. Arekal, Mohan kumar S, Keerthana H, Manoj kumar D, Muralidhar S, Priya R, Vijay kumar CB, Savitha Janakiraman