PHENOTYPICAL AND GENOTYPICAL ASSESSMENT OF RESISTANCE OF KLEBSIELLA PNEUMONIAE STRAINS PRODUCING
CARBAPENEMASE

Short Communication - Journal of Plant Biotechnology and Microbiology (2021) Volume 0, Issue 0

Lactic acid bacteria (LAB) are essential ingredients in probiotic foods, intestinal microflora, and dairy

products that are capable of coping up with harsh gastrointestinal tract conditions and are available in a

variety of environments. The objective of this study is to evaluate the probiotic property of LAB isolated

from bovine milk. Milk samples were collected from local dairy farms. Samples were obtained using

sterile test tubes and transported to a laboratory in the icebox for further biochemical characterization.

Preliminary Physiological and biochemical identification of LAB isolates was conducted by growing on

MRS agar after ten-fold serial dilution. Seven of the best isolates were selected for the evaluation of the

probiotic property. The LAB isolates were checked for resistance to antibiotics and their antimicrobial

activity by disc diffusion assay and agar well diffusion assay respectively. Bile salt hydrolase activity of

isolates were studied by growing isolates in BSH medium with bile salt. Cell surface property of isolates

was assayed by studying their autoaggregation and coaggregation percentage with S. aerues. All isolates

were found BSH positive. In addition, BCM2 and BGM1 were susceptible to all antibiotic disks except

BBM1 which was resistant to all antibiotic disks. BCM1 and BGM1 had the highest autoaggregation and

coaggregation potential respectively. Since all LAB isolates showed gastro intestinal tolerance and good

cell surface property they could be considered as good potential probiotic candidates for treatment and

probiotic starter culture preparation..

Author(s): Anna Samoilova, Ivan Likhachev, Elizaveta Rogacheva