Research Article - Biomedical Research (2020) Volume 31, Issue 3
Novel decellularized extracellular matrix scaffolds derived from porcine costal cartilages via a combination of supercritical carbon dioxide and acidic treatments.
Tissue-engineering was important and popular which combine medical applications and engineering materials knowledge, just like cell extraction process was employed to remove the cellular components from porcine costal cartilages, leaving a framework of largely insoluble collagen, elastin, and tightly bound glycosaminoglycans (GAGs) in this work. A cell extraction process of supercritical carbon dioxide (ScCO2) and acidic treatments was used to remove cellular components by porcine costal cartilages. Varying pore sizes and porosities of the acellular tissues were then created using enzymes and collagenase after acidic treatments. The porcine costal cartilages must remove lipids and other impurities almost by ScCO2, otherwise adipose tissue will become rot immediately. For collagen scaffold samples, porcine costal cartilages were hydrolyzed by enzymes and pH reagents, in this way it can gain state completely and clearly. Electron microscopy revealed effect in decellularized extracellular matrix (ECM) of porcine costal cartilages via supercritical fluid and enzymes after acidic treatments.Author(s): Ching-Cheeng Haang