Journal of Plant Biotechnology and Microbiology

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Short Communication - Journal of Plant Biotechnology and Microbiology (2021) Volume 4, Issue 2

Isolation, molecular identification and genomic pattern of Mycobacterium bovis isolates collected from tuberculin-positive cattle in infected farms of Shiraz

Fifty pathological samples from tuberculin-positive cattle collected from two slaughterhouses at Shiraz were subjected to bacterial culture on glycerinated and pyruvated Lowenstein-Jensen media. Genomic material from culture-positive slopes was extracted and used in PCR-16S rRNA, PCR-IS6110, and PCR-RD typing experiments. All the M. bovis isolates were further genotyped using pvu II-digested PGRS-RFLP strategy

Author(s): Sachiko Isobe

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