Research Article - Journal of RNA and Genomics (2021) Volume 17, Issue 0
Impact of glyphosate on SREBP-1c and expression in adipose tissue of male albino Wistar rats.
Glyphosate is used as an herbicide. At sub agriculture concentrations, glyphosate-based herbicide inhibits the proliferation. Peroxisome Proliferator Activated Receptor-? (PPAR-gamma) which is known as the master gene in adipogenesis has been inhibited by glyphosate-based herbicides. Sterol Regulatory Element-Binding Protein-1c (SREBP-1c) is a transcription factor involved in fatty acid metabolism and cholesterol which is stimulated by insulin. Glyphosate raises the environmental threat potential and also the public health concerns. Hence, we investigated the effect of glyphosate on the expression of SREBP-1c and PPAR-?. The study aims to determine the glyphosate-induced detrimental changes in SREBP-1c and PPAR-? mRNA expression in adipose tissue of adult male rats. Adult male Wistar albino rats were divided into 4 groups, each consisting of 6 animals. Group I served as normal control rats; Group II-IV consisted of glyphosate-exposed rats at different concentrations (50, 100, and 250 mg/kg body weight respectively) orally for 16 weeks. After 16 weeks of treatment, the animals were sacrificed and adipose tissue was dissected out for the assessment of SREBP-1c and PPAR-? mRNA by Real Time-PCR using gene-specific down-regulated primers. The data were analyzed statistically by a one-way Analysis Of Variance (ANOVA) followed by Duncan’s multiple range test was used to see the statistical significance among the groups. The results with the p<0.05 level were considered to be statistically significant. ) in the expression of SREBP-1c in all the glyphosate exposed rats compared to control rats and PPAR-? mRNA expression was found to be significantly reduced in a concentration-dependent manner (P<0.05) compared to normal control animals.Author(s): Rakshitha VS, Gayathri R, V Vishnu Priya, J Selvaraj, Kavitha S