Biomedical Research

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.
Reach Us +1-504-608-2390

- Biomedical Research (2015) Volume 26, Issue 2

HPLC Determination of Glycyrrhizic and Glycyrrhetinic acids in Weiyanning granule.

To establish a method for determination of glycyrrhizic and glycyrrhetinic acids in Weiyanning Granule. The contents of glycyrrhizic and glycyrrhetinic acids are determined by HPLC under conditions as follows: Diamonsil C18 (4.6 mm × 250 mm, 5 μm) column, mobile phase of acetonitrile- 0.05 mol/L ammonium acetate solution (A: 0.05 mol/L ammonium acetate solution; B: acetonitrile, gradient elution: 0~30 min: 32% B; 30~60 min: 75% B); flow rate of 1.0 ml/min; and detection wavelength of 250 nm. The determined linear range of glycyrrhizic acid is 0.320~1.920 μg, and average recovery is 98.6%, with RSD = 1.98%; linear range of glycyrrhetinic acid is 0.039~0.234 μg, and average recovery is 97.3%, with RSD = 3.20%. The method established in this paper is simple, accurate and reproducible, which provides a new way for the quality control of Weiyanning Granule.

Author(s): Shiqiong Zhou, Xiangyou Zhang, Xufei Duan, Hong Mei

Abstract Full Text PDF

Get the App