Research Article - Biomedical Research (2020) Volume 31, Issue 5
Evaluation of antibacterial and non-antibacterial adhesives in terms of cytotoxicity in cell culture medium
Purpose: The purpose of the study is to evaluate the adhesive systems containing antibacterial components (Clearfil SE Protect Bond-CPB, Peak Unıversal Bond-PUB, Gluma 2 Bond-G2B) and adhesive systems without antibacterial components (Clearfil SE Bond-CB, Gluma Self Etch Bond-GB) in terms of cytotoxicity in the cell culture medium.
Materials and methods: We compared the cytotoxic effects of five different adhesive systems in the cell culture medium by direct contact method. Four different dilutions (1%, 0.1%, 0.01%, 0.001%) of test materials were incubated in three different periods (24-48-72 hours) in the L929 mouse fibroblast cell culture medium and their effects on cell proliferation were evaluated with the XTT Tetrazolium Assay test.
Results: Comparing the cytotoxic effects of five different adhesive systems as time-dependent, the two highest concentrations of CB and CPB and only the highest concentration of GB showed significant cytotoxicity (P<0.05) in the 24-hour incubation. PUB showed cytotoxic effects at all concentrations tested. This effect has been shown to be dose-dependent. In 48-hour and 72-hour incubations, GB and PUB increased the cytotoxic effect in a dose-dependent manner (P<0.05). On the other hand, G2B, CB and CPB were found to be cytotoxic only at the two highest concentrations and the difference of CPB was more significant (P<0.001).
Conclusion: Antibacterial contents added to adhesive systems had no adverse effect on biocompatibility. The highest concentration caused the highest cytotoxic effect in adhesive systems with and without antibacterial components, which increased with incubation time.Author(s): Derya Turel Silsupur, Elif Pinar Bakir, Seyhmus Bakir