Biomedical Research

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.
Reach Us +1-504-608-2390

- Biomedical Research (2011) Volume 22, Issue 2

Effects of Smilax myosotiflora on testicular 11?-hydroxysteroid dehydro-genase oxidative activity and plasma hormone levels in rats

Smilax myosotiflora, a popular local aphrodisiac is known to increase sexual libido. How-ever, the aphrodisiac efficacy of S. myosotiflora has not yet been scientifically established. Present investigation describes the effects of S. myosotiflora on testicular 11β-hydroxysteroid dehydrogenase (11β-HSD) oxidative activity and the levels of plasma corticosterone and tes-tosterone. Male Wistar rats (200-250g) were given either 8 mg/kg BW/day of S. myosotiflora or 120 μg/kg BW/day of Mifepristone (RU486, a glucocorticoid receptor (GR) antagonist) or both together (RU486+S. myosotiflora), for seven consecutive days. Results between groups were analyzed using analysis of variance (ANOVA) and Student’s t test. Differences were considered significant at P<0.05. Rats given S. myosotiflora showed no significant changes in 11β-HSD activity but had increased corticosterone (P<0.01) and decreased testosterone (P<0.01) levels compared to controls. Administration of RU486 alone decreased 11β-HSD activity (P<0.001), but increased testosterone levels (P<0.05) compared to controls. Con-versely, rats with RU486+S. myosotiflora showed increased 11β-HSD activity (P<0.001) but decreased testosterone levels (P<0.05) compared to RU486-treated rats. In the rats treated with RU486+S. myosotiflora, none of the parameters differed significantly from controls. Plasma corticosterone levels were found to be lowered in rats treated with RU486+S. my-osotiflora compared to S. myosotiflora-treated rats (P<0.05) towards control values. In this study, S. myosotiflora at a dose of 8 mg/kg BW/day was found to increase corticosterone with a corresponding decrease in testosterone levels. Previous studies show that RU486 acts through GR in affecting 11β-HSD activity and hormone levels. Since RU486+S. myosotiflora rats show opposite effects from RU486 rats, we therefore suggest that the actions of S. my-osotiflora on these parameters are possibly mediated through the GR. S. myosotiflora and RU486 probably competitively inhibit each other at the GR level.

Author(s): Damayanthi D, Azman MAB, Aminuddin AHK, Hamid A and Nwe KHH

Abstract Full Text PDF

Get the App