Research Article - Journal of RNA and Genomics (2021) Volume 17, Issue 0
Effect of diosmin on the expression of matrix metalloproteinase-2 and-9 and p53 in the liver of N-Ndea-induced experimental rats.
Hepatocellular carcinoma most commonly occurs in people with liver disease, particularly in people with chronic hepatitis B and C. Diosmin is a type of plant derived chemical compound found mainly in citrus fruit. Understanding the advances of the molecular pathogenesis of HCC have led to identification of critical driver mutations, however the most prevalent of these are not yet druggable targets. Chemotherapeutic agent, an antineoplastic agent inhibits the uncontrolled proliferation of cancer cells in our body. In a previous study diosmin is used to treat hemorrhoids and thus has anticancer properties. To analyse the effect of diosmin on the expression of matrix metalloproteinase 2 and 9 and p53 in the liver of N-NDEA-induced experimental rats. Healthy male albino rats of wistar strain were divided into three groups with six animals each. mRNA expression of matrix metalloproteinase and p53 expression was studied in hepatocellular carcinoma induced animals treated with diosmin. The data were analyzed statistically by a one-way Analysis of Variance (ANOVA) followed by Duncan’s multiple range tests was used to see the statistical significance among the groups. The results with the p<0.05 level were considered to be statistically significant. Hepatocellular induced rats exhibited a significant increase in MMP2 and MMP9 and decrease in p53 marker. Treatment of NNDEA induced rats with diosmin shows a reversal in the levels of markers which was equivalent to the control groupsAuthor(s): Kavitha S, J Selvaraj, V Vishnupriya, Gayathri R