Research Article - Biomedical Research (2021) Volume 32, Issue 4
Comparative phytochemical assessment of Ashodhita and Shodhita seeds of Ipomoea carnea Jacq (An ethno pharmacological drug).
Background: Ipomoea carnea Jacq is an ethnopharmacological drug and its leaves, roots and latex are used for various indications by traditional healers. But seeds are not used in the treatment as they are more toxic. As the herbal medicines are becoming endangered, ethnopharmacological drug can be used efficiently as they are available abundantly. But the toxicity should be reduced for safer use in the treatment. To study the effect of Shodhana of Ipomoea carnea seeds and to study the physicochemical and phytochemical changes in seeds after shodhana in different media, the present study was undertaken.
Methods: Shodhana of 20 gm of Ipomoea carnea seeds was performed with Godugdha (Cow’s milk) with the help of method of swedana (boiling) in dolayantra for three hours. The same procedure was followed for another samples separately with Gomutra (Cow urine), Kanji (sour gruel), Triphala kwatha and Distilled water as shodhana media. One raw sample was kept as Ashodhita sample for comparison. Physicochemical and phytochemical analysis of all the six samples was conducted and the observations were compared among all the samples.
Results: The toxic principle Swainsonine was estimated as 3% in all samples at retention time 3 minutes. The number of peaks were reduced in Godugdha, Gomutra, Kanji and Triphala Kwatha Shodhita samples in comparison with Ashodhita sample and 2-4 peaks are observed at retention time 1-3 minutes in all shodhita samples. A new phytoamine having concentration 12% was detected at retention time 17 minutes in all (crude and purified) samples.
Conclusion: The number of peaks was reduced in shodita samples which may be due to antagonist action of shodhana media and 2-4 peaks are found in all shodhita samples which may be added due to the antidotal action of shodhana media. A new phytoamine detected at retention time 17 minutes in all samples needs to be identified and characterized using more advance tools like LC-MS or GC-MS.Author(s): Nilima Wadnerwar, Meena Deogade