Research Article - Journal of RNA and Genomics (2021) Volume 17, Issue 17

Chronic exposure of glyphosate modulates the expression of inflammatory signaling molecules in brain tissue in adult male rats.

Glyphosate is an active compound and non-selective widely used herbicide among farmers in all over developing countries. When glyphosate is applied to growing plants like broad leaf plants, weeds; it gets absorbed via tissue and kills the plants. Glyphosate -based herbicide exposed to rats causes oxidative stress. Glyphosate induces oxidative stress, leads to imbalance in production and accumulation of oxygen reactive species in cells and tissues which induce inflammation. To analyse the expression of inflammatory markers with chronic exposure of glyphosate among experimental rats. Total RNA isolation followed by conversion of RNA to cDNA with the help of reverse transcriptase and the mRNA expression level of TNFα, IL-1β was analyzed by RT-PCR. The triplicate statistical analysis results of the experiments performed on control and treated rats were expressed as mean ± SEM. Results were analyzed statistically by one-way Analysis Of Variance (ANOVA) and significant differences between the mean values were measured using Duncan's multiple range tests using Graph Pad Prism version 5. The results with p<0.05 level were considered to be statistically significant. Total RNA isolation followed by conversion of RNA to cDNA with the help of reverse transcriptase and the mRNA expression level of TNFα, IL-1β was analyzed byRT-PCR. The triplicate statistical analysis results of the experiments performed on control and treated rats were expressed as mean ± SEM. Results were analyzed statistically by one-way analysis of variance (ANOVA) and significant differences between the mean values were measured using Duncan's multiple range tests using Graph Pad Prism version 5. The results with p<0.05 level were considered to be statistically significant. Our present in vivo findings for the first time clearly demonstrate that glyphosate exposure leads to the development of diabetic neuropathy by modulating the expression of genes involved in the proinflammatory cytokines in the brain tissues. Further studies on the impact of glyphosate on downstream signaling molecules are warranted in order to ascertain the potential mechanisms of action of glyphosate.

Author(s): GV Venkatakarthikeswari, Gayathri R, V Vishnu Priya, J Selvaraj, Kavitha S

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