Objective: To explore the effect of Lycium barbarum L. on premature ovarian and the related mechanism.
Methods: The ingredients of Lycium barbarum L. were extracted with water and alcohol, respectively. Rats with premature ovarian were treated with water extractive and ethanol extractive, respectively. After treatment, the biological behaviors, weight of ovary and uterus of rats were determined and compared. The contents of E2, FSH, and LH were determined by ELISA. The protein expression values of PLA2G4A, PAPPA, STC2, CCL2 and NELL1 were determined by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and Western blotting, respectively.
Results: Both water extractive and ethanol extractive could improve the behaviors of rats. Water extractive could obviously increase the content of estrogen in blood and the size of uterine. Compared with ethanol extractive, water extractive could obviously regulate the expression of premature ovarian related genes.
Conclusion: Both water extractive and ethanol extractive have roles in treating rats with premature ovarian. Moreover, the treatment result of water extractive was above the ethanol extractive.