In this study, 32 osteosarcoma patients admitted in the department of orthopedics from 2008 to 2016 were selected as subjects. Percutaneous intra-arterial chemotherapy was performed on 17 patients on the day before admission, and then the general chemotherapy was carried out on next day. After limb salvage surgery, the previous chemotherapy was repeated for 4 times. Before limb salvage surgery, the remaining 15 patients were treated with intravenous systemic chemotherapy. Fourteen days later, amputation was performed. Both the treatment strategies were compared and it was concluded that patients can adapt better to the method of limb salvage treatment after arterial and venous chemotherapy, thus the application of which is more promising.