Biomedical Research

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Research Article - Biomedical Research (2017) Volume 28, Issue 14

Study on changes of Th1/Th2 cytokines with the treatment of Huang-Qin-Tang in damp-heat type ulcerative colitis rats

Objective: Explore the changes of Th1/Th2 cytokines with the treatment of Huang-Qin-Tang in dampheat type ulcerative colitis rat.

Methods: A total of 90 SD rats were randomly divided into blank group, model group and Huang-Qin- Tang group. Blank group were not given any drug intervention, while Huang-Qin-Tang group and model group were given high-fat high-sugar diet and trinitro-benzene-sulfonic acid in artificial of hot humid climate to induce damp-heat type ulcerative colitis rat model. The model group did not receive any treatment measures, while the Huang-Qin-Tang group used Huang-Qin-Tang. Clinical activity, changes in body weight, colon length weight, spleen and thymus weight and myeloperoxidase (MPO) activity of three groups were observed. Serum interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-10 (IL-10), interleukin-12 (IL-12) of rats was tested in the three groups.

Results: After treatment, the activity and the symptoms of Huang-Qin-Tang group were improved compared with model group. The expression of Th1 cytokines (IFN-γ, IL-12) decreased and Th2 cytokines (IL4, IL-10) increased in Huang-Qin-Tang group.

Conclusion: The Huang-Qin-Tang can effectively improve the inflammatory reaction of ulcerative colitis in rats and regulate the balance of Th1/Th2 cytokines, which provides a new idea and method for clinical treatment of damp-heat type ulcerative colitis.

Author(s): Chen Li, Liu Shi, Qian-De Zhang, Xiu Huang, Xu-Bo Wang, Qing Liu

Abstract Full Text PDF