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Study on changes of Th1/Th2 cytokines with the treatment of Huang-Qin-Tang in damp-heat type ulcerative colitis rats

Objective: Explore the changes of Th1/Th2 cytokines with the treatment of Huang-Qin-Tang in dampheat type ulcerative colitis rat.

Methods: A total of 90 SD rats were randomly divided into blank group, model group and Huang-Qin- Tang group. Blank group were not given any drug intervention, while Huang-Qin-Tang group and model group were given high-fat high-sugar diet and trinitro-benzene-sulfonic acid in artificial of hot humid climate to induce damp-heat type ulcerative colitis rat model. The model group did not receive any treatment measures, while the Huang-Qin-Tang group used Huang-Qin-Tang. Clinical activity, changes in body weight, colon length weight, spleen and thymus weight and myeloperoxidase (MPO) activity of three groups were observed. Serum interferon-γ (IFN-γ), interleukin-4 (IL-4), interleukin-10 (IL-10), interleukin-12 (IL-12) of rats was tested in the three groups.

Results: After treatment, the activity and the symptoms of Huang-Qin-Tang group were improved compared with model group. The expression of Th1 cytokines (IFN-γ, IL-12) decreased and Th2 cytokines (IL4, IL-10) increased in Huang-Qin-Tang group.

Conclusion: The Huang-Qin-Tang can effectively improve the inflammatory reaction of ulcerative colitis in rats and regulate the balance of Th1/Th2 cytokines, which provides a new idea and method for clinical treatment of damp-heat type ulcerative colitis.

Author(s): Chen Li, Liu Shi, Qian-De Zhang, Xiu Huang, Xu-Bo Wang, Qing Liu