Purpose of present research was to prospectively test in dog model of ARF with hypothesis that the CEUS can quantitatively evaluate the perfusion changes of renal cortex in the early stage of the disease. The 15 healthy adult ARF dog models were injected intramuscularly with 50% glycerin into the hind legs followed by i.v. bolus 0.2 ml Sonovue in leg vein. Real time samples from dogs were collected with conventional and CEU examinations. The Blood serum Urea Nitrogen (BUN) and Serum Creatinine (SCr) level were tested along with kidney tissue sample of randomly selected dog. The converted parameters and blood test were compared using statistical software. Initial values of slope of Ascending curve (A), derived Peak Intensity (PI), Time to Peak (TTP), Area under Curve (AUC) were significantly high than the values before injection. In histopathological analysis no significant change was observed in 6 h after the injection of glycerin (P>0.05) and might be seen after 24 h (P<0.05). Quantitative analysis of CEU could monitor the changes of blood perfusion image of the renal cortex of the early renal function changes of dogs than SCr and BUN, has been more and more used in the diagnosis of clinical disease.