Biomedical Research

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Research Article - Biomedical Research (2017) Volume 28, Issue 19

Pim-1 kinase regulates CD4+T cell differentiation

This study aimed to investigate the role of Pim-1 kinase in the regulation of naïve CD4+T cell differentiation. Naive CD4+T cells were isolated from the spleen of the mice and treated with different cytokines and Pim-1 kinase inhibitor. The expression of T-bet, GATA3, FOXP3 and RORγt proteins in naïve CD4+T cells was detected by Western blot analysis. The expression of cytokines IL-4, TGF-β, IFN- γ and IL-17 in supernatant was detected by ELISA. We found that the inhibition of Pim-1 kinase significantly reduced the expression of T-bet and ROR-γt during naïve CD4+T cells differentiation and inhibited the secretion of IFNγ and IL17 (P<0.05). In conclusion, our study suggests that Pim-1 kinase promotes the differentiation of naive CD4+T cells into Th1 and Th17 cells.

Author(s): Yueming Shen, Yuanhong Xie, Yan Zhao, Yan Long, Linqian Li, Ya Zeng

Abstract Full Text PDF