The present study aimed to evaluate the prevalence and antimicrobial susceptibility, of aerobic and anaerobic strains in 100 patients with head and neck (single and multiple) abscesses. In total, 114 microbes were isolated. The sensitivity test was performed for both aerobic and anaerobic isolates. Out of 100 patients (65 males, 35 females), 74 presented with single space abscess and 26 patients with multiple space involvement. The submandibular space was the most frequent location for single space and multiple abscesses (76.92%; 84.92%). Predominantly 48 aerobic and 65 anaerobic bacteria were isolated; the most common bacteria being Klebsiella, Clostridium tertium, Staphylococcus aureus and Actinomyces odontolyticus. The anaerobic bacteria were found to be 100% resistant to amoxicillin and penicillin, whereas they were found to be susceptible to erythromycin (100%), cefotaxime (98.48%) and clindamycin (96.96%) respectively. On the other hand, the aerobic strains were resistant to penicillin (47.91%) and sensitive to amoxicillin (79.16%) respectively. Hence, the antibiotics alone cannot determine the odontogenic infection satisfactorily. For immediate recovery of patients, proper basic management of the disease is also equally important.