Biomedical Research

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Research Article - Biomedical Research (2017) Volume 28, Issue 7

Lung injury following inhaled chemotherapy

Objective: We evaluated lung injury following inhaled treatment with paclitaxel, and determined the potential mechanism underlying the lung injury and remodeling following inhaled treatment.

Methods: Sprague-Dawley rats were treated with paclitaxel (3 mg/kg), via tracheal intubation and mechanical aeration. Histopathological changes in lung tissue were determined by haematoxylin and eosin staining. In addition, activity of matrix MMP-2 and MMP-9 was measured by gelatin zymography.

Results: Significant lung injury was found in paclitaxel-treated rats of ≥ 3 mg/kg group, P<0.05. In contrast, lung injury was mild in<3 mg/kg group. Activity of MMP-9 and MMP-2 in lung tissue was significantly increased in all rats received inhaled treatment, P<0.05. However, the magnitude of increase was greater in ≥ 3 mg/kg paclitaxel-treated group compared to<3 mg/kg groups. In addition, the increase of MMP-9 activity appeared to be time-dependently suppressed, whereas the increase of MMP-2 activity was time-dependent exacerbated.

Conclusion: Inhaled chemotherapy with paclitaxel at dose 3 mg/kg and over may lead to significant lung injury. MMP-9 may play important role in lung injury, whereas MMP-2 may play important role in lung remodeling following inhaled treatment.

Author(s): Ming Cai, Yuan Weng

Abstract Full Text PDF