Biomedical Research

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Research Article - Biomedical Research (2018) Volume 29, Issue 20

Inhibitory effect of PEG-5-Fu-MAMS combined with constant magnetic field on the growth of LoVo colon cancer cells in vitro

Objective: To investigate the inhibitory effect of polyethylene glycol modified magnetic 5-fluorouracil albumin microspheres (PEG-5-FU-MAMS) combined with external constant magnetic fields on LoVo colon cancer cells in vitro and the mechanism.

Methods: The PEG-5-FU-MAMS were prepared by emulsification/solidification method. The cultured LoVo cells were divided into four groups. Group A was PEG-5-FU-MAMS combined with external magnetic fields; group B was pure PEG-5-FU-MAMS without magnetic fields; group C was free 5- fluorouracil (5-FU); group D was pure magnet. The growth of LoVo cells were observed under an inverted microscopy and the inhibitory rate (IR) was assayed by methyl thiazol tetrazolium (MTT) assay.

Results: After different treatment, the tumor growth showed no difference on tumor cells in pure magnet group. The IR of pure PEG-5-FU-MAMS and free 5-FU at same drug concentration was similar, there was no difference between them. Compared with pure PEG-5-FU-MAMS and free 5-FU, when the concentration of 5-FU exceeded 10.0 mg/L, IR was increased after PEG-5-FU-MAMS was combined with constant external magnetic fields.

Conclusion: The growth inhibiting action was significantly enhanced after PEG-5-FU-MAMS combined with constant external magnetic fields.

Author(s): Jiangang Liu, Zhichao Liu, Keqiang Wang

Abstract Full Text PDF