Research Article - Biomedical Research (2018) Volume 29, Issue 2
Hypoxic microenvironment promotes proliferation and invasion of non-small cell lung cancer A549 Cells through Wnt/ÃÂ²-catenin signaling pathway
Objective: To study the effect of Wnt/β-catenin signaling pathway on hypoxia induced proliferation and invasion in lung cancer cell line A549.
Methods: Cultured lung cancer A549 cells were treated with hypoxia for 24 h. The proliferation rates were detected by CCK8; the mRNA and protein expression levels of HIF-1α and β-catenin were detected by RT-PCR and Western blot; the effect of hypoxia on invasion ability of A549 cells were detected with Transwell method. Furthermore, β-catenin siRNA was used to investigate the role of β-catenin on hypoxia induced proliferation and invasion.
Results: The results demonstrated that hypoxia could promote A549 cells proliferation and invasion. The mRNA and protein level of HIF-1α and β-catenin was upregulated by hypoxia treatment, whereas β- catenin siRNA could block these processes.
Conclusion: Hypoxia promotes osteosarcoma cell line A549 proliferation and invasion by activating Wnt/β-catenin signaling pathway.Author(s): Ruidong Ma, Xiaoping He, Hong Wang, Weikun Jia, Xiaofei Zeng