This study aimed to investigate the expression and clinical significance of HOX Transcript Antisense RNA (HOTAIR) in endometrial carcinoma, and discuss its correlation with transforming growth factor- β1 (TGF-β1) in EC tissue. Sixty patients with EC were enrolled. The EC tissue and cancer adjacent tissue (control) were collected. The expressions of HOTAIR and TGF-β1 mRNA in EC and adjacent tissue were determined using real-time quantitative PCR. Results showed that, the relative expression levels of HOTAIR and TGF-β1 mRNA in EC tissue were significantly higher than those in adjacent tissue (P<0.05). The expression of HOTAIR mRNA was significantly correlated with pathological grade, muscular invasion depth and clinical stage of EC (P<0.05), and that of TGF-β1 mRNA was significantly correlated with muscular invasion depth and clinical stage of EC (P<0.05). In EC tissue the expressions of HOTAIR and TGF-β1 mRNA were positively correlated (r=0.676, P<0.05). There was significant difference of overall survival of patients between high and low HOTAIR expression groups (χ2=4.346, P<0.05). The abnormal expression of HOTAIR may be involved in the occurrence and development of EC. The expression of HOTAIR is positively correlated with TGF-β1 expression in EC tissue.