The aim of this study is to demonstrate that percutaneous injections of autologous Adipose Tissue- Derived Stem Cells (ASCs) and Extracellular Matrix (ECM) in the form of adipose Stromal Vascular Fraction (SVF), along with Hyaluronic Acid (HA) and Platelet-Rich Plasma (PRP) activated by calcium chloride could regenerate cartilage-like tissue in human hip Osteoarthritis (OA) patient. Autologous lipoaspirates were obtained from adipose tissue of the abdominal origin. Afterwards, the lipoaspirates were homogenized to obtain ECM. This homogenized lipoaspirates were then mixed with collagenase and incubated. The ASCs/ECM-containing SVF was mixed with calcium chloride-activated PRP plasma and HA, and this ASCs mixture was injected into a hip of a patient with OA. Pre-treatment and posttreatment MRI T2 views, functional rating index, range of motion, and pain score data were analysed. The patient’s Magnetic Resonance Imaging (MRI) data showed significant positive changes. Cartilagelike tissue regeneration was evident in MRI studies of the patient. Along with MRI evidence, the measured physical therapy outcomes in terms of range of motion, subjective pain, and functional status, all improved. These results indicate that percutaneous injection of the ASCs mixture is a promising minimally invasive therapy for OA of human hip.