Biomedical Research

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Research Article - Biomedical Research (2017) Volume 28, Issue 9

Effects of berberine on the expressions of NRF2 and HO-1 in endothelial cells of diabetic rat

Objective: This study aims to investigate the effects of berberine on the expression of nuclear factor erythroid 2-related factor 2 (Nrf2)/Heme Oxygenase 1 (HO-1) in retinal cells of diabetic rats.

Methods: The diabetic rat models were established through intraperitoneal injection of streptozotocin. Different concentration (50 mg/kg/d, 100 mg/kg/d, 200 mg/kg/d) of berberine was orally administered to the rats for 4 weeks. After 4 weeks, changes of body weight, blood lipid and glucose were detected, and the expression levels of Nrf2 and HO-1 in retinal tissues were evaluated by immunohistochemistry and Western blot.

Results: Four weeks of berberine intervention decreased blood glucose to below 16 mmol/L. Total Cholesterol (TC), Triglyceride (TG), and Low-Density Lipoprotein cholesterol (LDL) was decreased significantly, while High-Density Lipoprotein cholesterol (HDL) was increased significantly than that in the model group (P<0.05). The expressions of Nrf2 and HO-1 were also decreased in retinal cells after berberine treatment compared with those in the model group.

Conclusion: Berberine can reduce blood glucose level in diabetic rats, improve blood lipid and decrease retinal vascular injury, suggesting it may be associated with the reduced expressions of Nrf2/HO-1.

Author(s): Kai Tao, Jinghai Chen, Lingyun Wang

Abstract Full Text PDF