Pollution of the water bodies will create physiological stress on the aquatic fauna. Among various forms of pollution in water bodies, detergent pollution has an immediate and detrimental effect. The floor cleaners are household chemical cleaning compounds required in wide range for diverse purposes. In some rivers the concentrations of cleaning products is quite high. The major components of cleaning products such as Surfactants make adverse effects on the aquatic life at very low levels of exposure. Effect of floor cleaners on the consumption of dissolved oxygen by Carassius auratus were determined by using Winkler’s method. The floor cleaners used were Lizol, dettol and lemon grass oil. This study demonstrated that dissolved oxygen consumption in Carassius auratus increases in the presence of floor cleaners. This is due to the stress caused by the floor cleaners on the normal physiological activities of the fish. Effect of floor cleaners on the respiratory rate of Carassius auratus were determined by counting its gill movements. This study demonstrated that there will be changes in ventilation rates of Carassius auratus when dissolved oxygen levels change. Thus draining of domestic sewage containing detergents into water bodies adversely affect its aquatic fauna. The project is selected because of the higher pollution nowadays. The results of the project conducted shows that while adding detergent dissolved oxygen level started decreasing with higher concentrations. Results show that Lizol shows a higher range of decrease in the dissolved oxygen level in water with higher concentration. And lemon grass oil shows less effect compared to other two floor cleaners. So, it is better to use natural cleaners for floor cleaning purposes.