Biomedical Research

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Effect and mechanism of laminaria japonica polysaccharide (LJP) on apoptosis and cycle of nasopharyngeal carcinoma cells

Objective: To explore the growth inhibition and apoptosis induced by Laminaria Japonica Polysaccharides (LJP) in human nasopharyngeal carcinoma cell lines.

Methods: Laminarin in vitro was adopted to affect human nasopharyngeal carcinoma HONE1 and CNE2; Cell proliferation curve, cell invasive ability, cell cycle changes and apoptosis related genes Bax and Bcl-2 were detected respectively by MTT assay, Transwell experiment, flow cytometry and western blotting.

Results: With Laminaria polysaccharide, the growth of human nasopharyngeal carcinoma cell line HONE1 and CNE2 was inhibited, invasive ability of cell decreased and the cell cycle of Raji cells was blocked in G0/G1 phase with down-regulation of Bcl-2 expression and up-regulation of Bax.

Conclusion: Laminaria japonica polysaccharide can regulate the apoptosis related genes Bax and Bcl-2 through the PI3K/AKT/mTOR pathway to promote the apoptosis of nasopharyngeal carcinoma cells in the G0/G1 phase.

Author(s): Fugui Wei, Xunxun Lu, Ying Lin, Min Guo, Wenzhong Sun