Advances in Cell Science and Tissue Culture

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Direct and indirect shoot organogenesis strategies for propagation and conservation of Abutilon ranadei Wooder & Stapf - A critically endangered plant.

The study aimed to evaluate the efficiency of direct and indirect organogenesis for large-scale multiplication, propagation and conservation of Abutilon ranadei, an endemic and critically endangered plant. Nodes and leaves from in vitro germinated seedlings were used as source of explants for direct and indirect shoot organogenesis respectively. High frequency shoot organogenesis (77.5%) with 7.1 ± 0.2 shoots were achieved when nodes were cultured on MS medium with BAP (3.0 mg/l) and GA (0.5 mg/l). The best callus induction and proliferation response was achieved with 2,4-D (2.0 mg/l) and BAP (1.5 mg/l). Maximum response (92.5%) for shoot regeneration from callus with 13 ± 0.8 number of shoots were obtained in MS medium fortified with KN (1.5 mg/l) and NAA (0.4 mg/l). Microshoots regenerated via direct as well as indirect organogenesis were rooted best in ½MS containing 1.5 mg/l IBA and 1.0 mg/l BAP. The well rooted plantlets were successfully established in the soil.

Author(s): Patil SS, Mane JJ, Umdale SD, Chavan JJ