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Determination of acarbose in tablets by attenuated total reflectance Fourier transform infrared spectroscopy

Attenuated total reflectance Fourier transform infrared (ATR- FTIR) spectroscopy method was developed for the quantitative determination of acarbose in tablets. Two calibration curves were generated. The first calibration curve was based on peak height at 2928.54 cm-1 with correlation coefficient of 0.9987. The second calibration curve was based on peak area which range from 2953.39-2851.04 cm-1 with correlation coefficient of 0.9978. The FTIR method was validated according to International Conference on Harmonization (ICH) guidelines. The method was precise over a range of 40%-100% w/w with % RSD values less than 2 for intra-day and interday precision. The accuracy of the method was expressed as the percentage recovery of the spiked acarbose standards with a mean recovery of 106.9%. The limit of detection and limit of quantification values were 0.004% and 0.012 % w/w, respectively. The validated method was used for the quantification of acarbose in tablets with good results.

Author(s): Ali Saoud, Gabriel Akyirem Akowuah*, Omotayo Fatokun, Ahmad Mariam and Shaik Ibrahim Khalivulla