Biomedical Research

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Assessment of biochemical parameters after exposure to ultrasound waves; an in vivo study

Introduction: The main aim of this study was to determine the effects of ultrasonic waves on serum levels of lactate dehydrogenase, aspartate transaminase, alanine transaminase, creatine phosphokinase and alkaline phosphatase in male rats. Normally, Serum level of LDH, AST, ALT, CK and ALP enzymes are influenced by environmental factors. These enzymes commonly measured as biomarkers for life health.

Methods: In this study male wistar rats were randomly divided into two groups, control and exposed to ultrasonic waves for 1, 6 h/day. Blood samples were obtained using cardiac puncture method. Following serum preparation, biochemical levels were measured. Data were statistically analysed and compared between groups using ANOVA and student t-test.

Results: The results indicated that serum level of ALT and LDH has a significant decrease. No differences were seen in the level of the serum of AST, ALP and CK enzymes in both the 1 h/day and 6 h/day of exposure group. And also serum level of ALT that has exposed 1 h/day comparing to control group didn’t have significant changes.

Conclusion: Exposure to ultrasonic waves may lead to side effects. These effects can be therapeutic effects for a range of patients and can also be very deadly for a group of patients with unbalanced serum levels of special enzymes.

Author(s): Ali Salimi, Rahim Ahmadi, Jamil Kheirvari Khezerloo