Biomedical Research

- Biomedical Research (2011) Volume 22, Issue 1

Anti-proliperative and antioxidant effects of Tinospora crispa (Batawali)

T. crispa is a well known traditional medicinal plant used in India, Indonesia, Philippines and Malaysia. To date, there are numerous studies on this plant emphasizing it’s antioxi-dant, antidiabetic, antimalarial and cosmetic effects. However, limited informations are available regarding T. crispa cytotoxicity potential. The objectives of this study were to de-termine the cytotoxicity potential and antioxidant activity of different T. crispa crude ex-tracts. Viability of cells was measured using the MTT assay on MCF-7, MDA-MB-231, HeLa and 3T3 normal fibroblast cells while antioxidant activity was determined by measur-ing total flavonoid content, total phenolic content and DPPH free radical scavenging activ-ity. Results obtained showed that the cell viability decreased in a dose-dependent manner in all cancer cells for each extract. The lowest IC50 (33.75 ± 4.65 μg/ml) was observed in MCF-7 using the methanol extract. Further more, there were significant differences for total pheno-lic content (p<0.01, df=2, X2=9.836) and flavonoid content (p<0.05, df=0.02, X2= 7.20) be-tween each extract with the methanol extract having the highest activity for both phenolic (255.33 ± 10.79 mg GAE/g sample) and flavonoid content (9.53 ± 0.50 mg QE/ g sample). The DPPH assay showed that the methanol extract had highest scavenging activity in a dose-dependent manner where the IC50 value was 12 μg/ml. T. crispa has a dose-dependent antiproliferative activity against many types of cancer cells where the lowest IC50 was pre-sent in the methanol extract on MCF-7. Further more, methanol crude extract of T. crispa had higher total phenolic and flavonoid content and free radical scavenging activity com-pared to water extract and chloroform extract.

Author(s): Ibahim MJ, Wan-Nor I?zzah WMZ, Narimah AHH, Nurul Asyikin Z, Siti-Nur Shafinas SAR, Froemming GA

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