Biomedical Research

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AB-PAS as a potential alternative for the diagnosis of intestinal metaplasia

Background: This study was to investigate the role of AB-PAS in the diagnosis of Intestinal Metaplasia (IM) as compared to HE staining, and to evaluate the relationship between Helicobacter pylori (H. pylori) infection and IM to explore whether IM was a possible pathogenic mechanism of H. pylori in gastric carcinogenesis.

Methods: Patients who suffered from Chronic Atrophic Gastritis (CAG) to Gastric Cancer (GC) were recruited, and the gastric tissues (CAG tissues and cancer tissues) were collected for HE staining, ABPAS and silver staining for detection of IM and H. pylori infection.

Results: Twenty-six patients meeting the inclusion criteria were recruited into the present study and a total of fifty-two slices were available. Thirty-four slices were IM (+) in HE staining, while forty-three slices were IM (+) in AB-PAS. The correlation coefficient between H. pylori infection and IM was about 0.41 (P<0.05).

Conclusions: AB-PAS is superior in the diagnosis of IM to HE staining and IM might be one of pathogenic mechanisms underlying the H. pylori induced gastric carcinogenesis.

Author(s): Sheng Quan, Jinping Lin, Zhinong Jiang, Xingkang He, Chen Sun, Zhenghua Lin, Tingting Su, Shujie Chen, Jianmin Si