The cardiotoxicity induced by doxorubicin are often studied by conventional techniques which are expensive and the experiment ends with the death of cells in culture. Thus a new impedance technique is needed to study the cardiotoxicity by overcoming the drawbacks of conventional techniques. The present paper discusses the measurement of cytotoxicity of doxorubicin on cardiomyocytes of neonatal rats by monitoring the impedance of cells cultured on impedance sensing device. The device was purchased from Applied Physics to perform the impedimetric cytotoxic study of doxorubicin on cardiomyocytes. The impedance of drug treated sample above 4 μM dose decreases rapidly due to fast cell death and detachment from the electrode surfaces. The impedimetric cytotoxic data compare well with the data of conventional techniques such as MTT assay and phase contrast imaging.