+1 (629)348-3199Wharton’s jelly mesenchymal stem cells and Insulin effect on BDNF expression in CA1 and CA3 regions of rats’ hippocampus after Chronic Hypoxia
18th International Conference on Neurology and Neurological Disorders
August 23-24, 2018 | Paris, France
Simin Mahakizadeha, Mohammad Akbaria, Mohammad Sharifzadehb, Tayebeh Rastegara, Farid Abolhassania and Gholamreza Hassanzadeha
Tehran University of Medical Sciences, Iran
Scientific Tracks Abstracts : J Neurol Neurorehabil Res
Abstract:
Objectives: Brain is vulnerable to deprivation of oxygen
supply during hypoxia, and therefore undergoes
neurodegeneration and cognitive dysfunction. Regarded
to Regenerative capacities of Wharton’s jelly -MSCs and
insulin at the site of injury, we were aimed to evaluate the
effect of Wharton’s jelly -MSCs and insulin on degenerative
consequences induced by chronic hypoxia.
Methods: 36 male rats were randomly divided in 6groups:
Control(C), Sham1-saline (Sh1), Sham2-surgery (Sh2),
Hypoxia (H), Hypoxia+ Insulin (HI), Hypoxia+ MSCs (HCs).
Animals were exposed to hypoxic chamber (8% O2, 92% N2)
for 30 days (4hours/day) in H, HI and HCs groups. Intranasal
insulin and stereotaxical MSCs in HI and HCs was used,
respectively. Spatial learning and memory were analyzed
using the Morris water maze task. BDNF gene expression
was studied in the hippocampus by real time-PCR.
Results: BDNF had the significant depletion in HI group and
magnification in HI and HCs groups comparing with C and
Sh groups (p < 0.05). Insulin and MSCs improve Hypoxia’s
signs such as BDNF gene expression fallen and memory
impairment.
Conclusions: In conclusion, we indicated that use of
insulin hormone and MSCs as neuroprotective and
stimulating factors for neurogenesis, could be beneficial in
neurodegenerative damage induced by hypoxia.
Biography:
Simin Mahakizadeha has completed her PhD from Tehran University of Medical Sciences, Iran and received her master’s degree in Anatomy from Golestan University of Medical Science, Iran. Her Bachelor’s degree was received from Shiraz University of Medical Sciences in the field of physiotherapy. She has published her papers and participated in many national and international conferences.
E-mail: si.mahakizadeh@gmail.com
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