+1 (629)348-3199Role of mir-185 and SEPT5 Genes in Pathogenesis of Parkinson’s disease in animal model
18th International Conference on Neurology and Neurological Disorders
August 23-24, 2018 | Paris, France
Kambiz Hassanzadeh and Arman Rahimmi
Kurdistan University of Medical Sciences, Iran
Posters & Accepted Abstracts : J Neurol Neurorehabil Res
Abstract:
Backgrounds and aim: The pathophysiology of Parkinson’s
disease (PD) has been under immense investigations for
more than a century. However, there are still many unknown
mechanisms toward the disease. Clarifying the molecular
mechanisms involved in PD, can assist in developing novel
and efficient therapies. Recently, studies showed genes
located on the human chromosome location 22q11.2 might
be involved in development of PD. Therefore, our research
aimed to evaluate the role of two genes located on the
chromosomal location (mir-185 and SEPT5) which were
the most probable candidates based on our bibliography.
Methods: Male wistar rats were divided in two groups,
randomly (n = 8). One group received rotenone injections
(1.5 mg/day s.c.) for 45 consecutive days. The other group
(control) only received rotenone injection vehicle (sunflower
oil). Behavioral tests including rotarod, rearing and bar tests
were performed at baseline and 45th day. Substantia nigra
and striatum were extracted from the animals’ brain. The
expression of mir-185 and SEPT5 genes were measured
at mRNA level using syber green real-time PCR technique.
Results: the results of behavioral tests showed significant
decrease in performance of rotenone treated group compared
to control group (P< 0.05). The level of mir-185 and SEPT5
genes also decreased significantly in substantial nigra of
rotenone treated group compared to control group (P< 0.05).
However, there was no significant difference between two
groups in expression of mir-185 and SEPT5 genes (P< 0.05).
Conclusion: the results of current study introduce mir-185 and
SEPT5 genes as novel genes participating in pathophysiology of PD.
Biography:
E-mail:
kambizhassanzadeh@gmail.comPDF HTML