PHYTOCHEMICAL PROFILE AND BIOLOGICAL PROPERTIES OF PISTACIA VERA L HULL ESSENTIAL OIL
Joint Event on 7th International Conference and Exhibition on PHARMACOLOGY AND ETHNOPHARMACOLOGY & 5th GLOBAL PHYSIOTHERAPY, PHYSICAL REHABILITATION AND SPORTS MEDICINE
March 27-28, 2019 | Amsterdam, Netherlands
University of Messina, Italy
Scientific Tracks Abstracts : Asian J Biomed Pharmaceut Sci
Although the chemical composition and biological properties of some species of the genus Pistacia has been investigated, studies on hull essential oil of Pistacia vera L variety Bronte (HEO) are currently lacking. In this work, we have carried out an in-depth phytochemical profile elucidation by Gas Chromatography-Flame ionization and Mass Spectrometry (GC-FID and GC-MS) analysis, and an evaluation of antioxidant scavenging properties, using several in vitro methods, and checking its cytoprotective potential on lymphocytes treated with tert-butyl hydroperoxide. Moreover, the antimicrobial activity against standard and clinical Gram-positive and Gram-negative as well as Candida sp. strains, was also investigated. Quali-quantitative analysis highlighted the richness of this complex matrix, with the identification of 40 derivatives. The major components identified were 4-Carene (31.743%), α-Pinene (23.584%), D-Limonene (8.002%), and 3-Carene (7.731%). The HEO showed a strong iron chelating activity and was found to be markedly active against hydroxyl radical. Moreover, HEO pre-treatment increase significantly the cell viability, decreasing the lactate dehydrogenase (LDH) release. HEO was bactericidal against all the tested strains at the concentration of 7.11 mg/mL, with the exception of Pseudomonas aeruginosa ATCC 9027, and fungicidal at concentrations between 2.50 and 5.0 mg/ml. The obtained results demonstrate the strong free-radical scavenging activity of HEO along with remarkable cytoprotective and antimicrobial properties, which makes the HEO potentially useful, particularly, in the treatment of fungal infections, especially drug-resistant ones.
Antonella Smeriglio is a research fellow in pharmacognosy at the department of chemical, biological, pharmaceutical and environmental sciences of the University of Messina, Italy. She has master’s degree in pharmacy, PhD in toxicology and specialized in pharmacognosy.
E-mail: [email protected]