44 7897 074717PHARMACOKINETIC DETERMINATION ATENOLOL ENANTIOMER IN RABBIT PLASMA BY REVERSE PHASE UFLC TECHNIQUE
Joint Event on GLOBAL PHARMA SUMMIT & 2nd International Conference on GASTROENTEROLOGY AND HEPATOLOGY
November 23-24 , 2018 | Bangkok, Thailand
BM Gurupadayya and Charan Raju C
JSS Academy of Higher Education and Research, India
Scientific Tracks Abstracts : Asian J Biomed Pharmaceut
Abstract:
The present work describes the pharmacokinetic profile of (R)- and (S)- Atenolol (ATL) enantiomer in rabbits using rapid and selective liquid chromatography with liquid-liquid extraction (LLE) method. The ATL enantiomers were extracted from plasma by means of LLE using and were analyzed on a Lux cellulose i5 segment (150×4.6 mm, 5μ) column with ultraviolet detection at 225 nm. Atenolol enantiomers indicated great determination with a retention time (tR) of 2.7 min and 3.10 min individually. The lower limit of quantification of the ATL enantiomers in plasma was 2 μg/ml. The validated method was successfully applied to chiral pharmacokinetic studies of oral administration of racemic ATL to rabbits. (S)-ATL showed almost similar AUC, Tmax, and Cmax and same half-life compared (R)-ATL, indicating similar bioavailability of the both isomer.
Biography:
BM Gurupadayya working as the professor in the department of Pharmaceutical chemistry at JSS College of Pharmacy, JSS Academy of Higher Education & Research, Mysuru, India. The current area of his interest lies in the field of bioanalytical method developments, chiral drug analysis and drug and herbal drug interactions. He has authored and co-authored over a hundred research papers on pharmaceutical analysis and pharmaceutical chemistry. He was awarded Dr PD Sethi Annual award’ for best research paper on application of TLC/HPTLC. I have received several research grants from AICTE, UGC, VGST and DST funding agencies. He has guided more than 30 students in their master’s program and 7 PhD degrees.
E-mail: bmgurupadayya@jssuni.edu.in
PDF HTML