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BIOLOGICAL EFFECTS OF EF24, A CURCUMIN DERIVATIVE, ALONE OR COMBINED WITH MITOTANE IN ADRENOCORTICAL TUMOR CELL LINES
Joint Event on 7th International Conference and Exhibition on PHARMACOLOGY AND ETHNOPHARMACOLOGY & 5th GLOBAL PHYSIOTHERAPY, PHYSICAL REHABILITATION AND SPORTS MEDICINE
March 27-28, 2019 | Amsterdam, Netherlands
Raffaele Pezzani, Loris Bertazza, Susi Barollo, Maria Elena Mari, Irene Faccio Maira Zorzan, Marco Redaelli, Beatrice Rubin, Decio Armanini and Caterina Mian
University of Padova, Italy Associazione Italiana per la Ricerca Oncologica di Base, Italy Venetian Institute for Molecular Science and Experimental Technologies, Italy
Posters & Accepted Abstracts : Asian J Biomed Pharmaceut Sci
Abstract:
Background: Curcumin is a polyphenol extracted from the plant Curcuma longa L. It has numerous properties
and is used in many preclinical conditions, including cancer. Curcumin has been tested in colorectal,
lung, breast, liver and many others tumor cell lines. It is known that curcumin has low bioavailability, while its
derivative EF24 showed enhanced solubility. However, its effects have been never explored in adrenocortical
tumor cell models.
Aim: This work analyzed the efficacy of EF24, a curcumin derivative, in 2 adrenocortical tumor cell line models,
SW13 and H295R.
Results: EF24 reduced cell viability by MTT with IC50 of 6.5 ± 2.4 μM and 4.9 ± 2.8 μM for SW13 and H295R
cells, respectively. Combination index (EF24 associated with mitotane) suggested an additivity effect in both
cell lines. Cell cycle analysis revealed an increase of subG0/G1 phase, while motility assay showed a decrease in
migratory cell capacity after drug treatment and similarly clonogenic assay indicated that EF24 (alone or combined
with mitotane) could reduce colonies number. Also, Wnt/β-catenin, NF-κB, MAPK and PI3k/Akt pathways
were modulated by western blot analysis when treating cells with EF24 alone or combined with mitotane.
Conclusions: This work analyzed for the first time a derivative of curcumin, EF24, in adrenocortical tumor cell
lines. These results suggest that EF24 could potentially impact on adrenocortical tumors, laying the foundation
for further research.
Biography:
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