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Journal of Microbiology: Current Research | Volume 2
November 01-02, 2018 | London, UK
7
th
European
Clinical Microbiology Congress
4
th
International Conference on
Ophthalmology and Eye Disorder
Joint Event
&
The structure, function and stability of the sinonasal microbiome during health and chronic
rhinosinusitis
Brett Wagner Mackenzie
The University of Auckland, New Zealand
C
hronic rhinosinusitis (CRS) is characterized by persistent
inflammation of the sinonasal mucosa. The condition
is highly prevalent (~5%) causing significant morbidity and
considerable treatment-associated costs to healthcare
systems. CRS is a complex, heterogeneous disease resulting
from interactions between a patient’s genetics, mucosal
immune system and sinonasal microbiome. The pathogenic
role of the microbiome in CRS remains incompletely
understood. With the aim of providing a context for CRS-
related microbiome research, I will present results from a
variety of traditional culture-based andmolecular techniques
that we have employed to explore the roles of both individual
bacterial strains and microbial communities. Amplicon
sequencing of the bacterial and fungal communities has shed
light on the dysbiotic, fragmented CRS bacterial community
and the natural temporal variability in healthy subjects.
Shotgun metagenomic sequencing was used to provide total
microbial community information, revealing the presence
of bacteriophages and bacterial strain-level diversity in
patients with CRS. I will highlight the substantial challenges
associated with metagenomic shotgun sequencing in low
biomass sinonasal samples and describe the advantages of
such an approach. This research contributes to our current
understanding of the role of the microbiome in CRS and will
help inform multifaceted, cross-disciplinary studies that aim
to develop more effective treatments for CRS.
Speaker Biography
Brett Wagner Mackenzie recently finished her PhD at the School of Medicine, Department
of Surgery at The University of Auckland in Auckland, New Zealand. Her thesis focuses on
the role of the human sinonasal microbiome in chronic rhinosinusitis and how to better
understand the role of the sinonasal microbiome during health and disease. Her research
interests include host-microbiome interactions and microbial community network stability
and response to disturbance.
e
:
bc.wagner@auckland.ac.nzBrettWagner Mackenzie, Clinical Microbiology and Eye 2018, Volume 2
DOI: 10.4066/2591-8036-C1-002