Journal of Fisheries Research

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Research Article - Journal of Fisheries Research (2022) Volume 6, Issue 4

Some morphometric and meristic characteristic of hybrid from albino clarias and normal clarias gariepinus (Burchell, 1822)

Apollos Garba T*

Department of Fisheries and Aquaculture, Adamawa State University Mubi, Adamawa State, Nigeria

*Corresponding Author:
Apollos Garba T
Department of Fisheries and Aquaculture
Adamawa State University Mubi
Adamawa State, Nigeria
E-mail: [email protected]

Received: 06-Jun-2022, Manuscript No. AAJFR-22-65937; Editor assigned: 08-Jun-2022, PreQC No. AAJFR-22-65937(PQ); Reviewed: 11-July-2022; QC No. AAJFR-22-65937; Published: 18-July-2022, DOI:10.35841/aajfr-6.4.116

Citation: Apollos GT. Some morphometric and meristic characteristic of hybrid from albino clarias and normal clarias gariepinus (Burchell, 1822). J Fish Res. 2022;6(4):116

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Abstract

A research was conducted to determine the morphometric and meristic characteristic of hybrid (cross between normal Clarias and albino Clarias through artificial breeding using the following matting combination. Clarias gariepinus Albino male (?) x Clarias gariepinus albino female (?), Clarias gariepinus albino female (?) x Claris gariepinus normal male (?), Clarias gariepinus normal male (?) x Clarias gariepinus albino female (?) and normal Clarias gariepinus male (?) x normal Clarias gariepinus female (?). Ten (10) morphometric and meristic characters were examined in samples from each mating combination. A total of 40 fishes were considered. Total length were measured using measuring board, weight were taken using sensitive weighing balance and others parameters were evaluated to the nearest 0.1 mm with digital Vanier caliper. The meristic count were measured using hand lens and a dissecting microscope. From the result obtained, the intermediate morphological traits of the two different coloured hybrids suggest that they are product of true fission of the genome of two different coloured fishes, except for some of the hybrid especially the reciprocal hybrids of female albino and normal male. All the morphometric and meristic characters are the same with their parent. The crosses of normal pigmented and albino Clarias gariepinus produces normal pigmented heterozygote with white and black patches on the body. Combine in the offspring and the offspring simultaneously demonstrates both parent phenotypes. The hybrid of female albino and normal male (AA? x NN?), male albino and female normal (AA? x NN?) had brownish colour phenotypically. Therefore, this external features characteristic of both male and female used for each hybridization exercise seemed to have little to no influence to the external features of the resulting hybrid

Keywords

Clarias gariepinus, Albino Clarias, Morphometric, Meristic count and Mubi.

Introduction

Morphometric and meristic analyses are part of important rigorous tools used to differentiate closely related species of organism having huge similarity indices of various parameters [1]. Morphometric characters are not only essential to the understanding of the taxonomy but also the health of a species as well as its reproduction in an environment. The shape and structures are unique to each species and the variations in its features are probably related to the habit and habitat among the variant of the species [2]. Although morphological characteristics are phenotypically plastic and are influenced seasonally by the physical environment factors during spawning and early juvenile stages of their life [3]. Morphometric assessment of fish species determines the inter relation between the body parameters like length, weight, fecundity and so on. It is also helps in the understanding of the relation between body parts [2]. Morphometric assessment is also used in the identification of the differences in fish population [4,5]. Morphometric variation between stocks can form one of the bases for stock structure and may be applicable for studying a short-term, environmentally induced variation geared towards successful fisheries management [6]. These measurements are restricted to document the direction of the size of variation in fish stock. The measurement is believed to be a suitable technique for the recognizing the degree of reproductive maturation without sacrificing the fish [7].

Generally, species of fish that have different origin is morphologically differentiated from each other. According to the reported works of analysis of phenotypic differences in morphometric characters or meristic counts is the method most commonly used to delineate stocks of fish [8-10]. According to Onyia this is often being used in discrimination and classification studies by statistical techniques but despite the advent of techniques which directly considers the biochemical or molecular genetic variation, these conventional methods still play vital functions in stock identification even to date [8]. The general health of a population of fish can be accessed through the growth features. So possible variations in the measurable and countable characters will reveal the adaptation to environmental condition, crossing viability and help in clarifying their identity [11]. There has not been any previous documented morphological description of the interspecific hybrids of these species. On this background, it is necessary to determine the morphometric and meristic characteristic of cross between albino and normal Clarias.

Materials and Methods

Offspring from mating combination of normal Clarias and Albino Clarias were obtained through artificial breeding of the following: Clarias gariepinus Albino male (♂) x Clarias gariepinus Albino female (♀), Clarias gariepinus Albino female (♂) x Clarias gariepinus normal male (♀), Clarias gariepinus normal male (♂) x Clarias gariepinus Albino female (♀) and Normal Clarias gariepinus male (♂) x Normal Clarias gariepinus female (♀).

Hatchlings from this mating combination were cultured for the period of one to determine the morphometric and meristic character. Ten (10) Morphometric and meristic characters were examined in samples from each mating combination. A total of 40 fishes were examined. The morphometric characters were measured using the conventional method described by Hubbs and larger [12]. The characters examined are: Total Length Standard Lengths, Weight, Head Width, girths, eye diameter, inter orbital distances, nasal barbel lengths, and maxillary barbell length. The other Morphometric character are dorsal fin length, dorsal fin height, caudal peduncle length, gap between adipose and dorsal fins, and fin length, and fin height, pectoral fin to pelvic fin, pelvic fin to anal fin, frontal frontanelle length, and occipital frontanelle width. Total length were measured using measuring board, weight were measured using sensitive weighing balance and others parameters were measured to the nearest 0.1mm with digital Vanier caliper, the meristic count were measured using hand lens and a dissecting microscope. The characters counted includes dorsal fin rays, pectoral fin rays, pelvic fin rays, and fin rays and caudal fin rays. All data obtained were subjected to one way analysis of variance (ANOVA) (SAS), while mean were separate using fisher LSD.

Results and Discussion

The result of the measurement are shown in table 1. The low mahalanobis square distance between the two different pigmented Clarias species indicated their level of similarities to each other. The mahalanobis is square distance (D2) between male albino and female normal (AA ♂ x NN ♀) female albino and male normal (NN♂ x AA♀), Albino male and Albino female. (AA♂ x AA♀), and normal male and female (NN♂ x NN♀) C. gariepinus including their offspring in f1 of the interspecific hybrid were not significantly different (p<0.05). This clearly indicate how extremely difficult it could be to distinguish this species from their interspecific hybrid using their morphological and meristic features. There are varying pattern of inheritance of some character by offspring of the various mating combination, the hybrid for the offspring of mating combination of normal and albino in F1 shows positive heterosis in the inheritance of brown eyes colour, head width, premaxillary width, and vomemine width in which case they possess different body color (brown) compared to the both parental width pink and black colored eyes. The head of all the mating combination shows the same flattered like the positive parent of C. gariepinus species. Therefore, this external features characteristic of both male and female used for each hybridization exercise seemed to have little to no influence to the external features of the resulting hybrid offspring except for the brown eyes and brown body observed in some offspring at the end of the research.

Mating combination Number sampled Head width Eye diameter Occipital fontanelle width Frontal fontanelle length Pre maxillary width Vomerine length Vomerine  width Pelvic fin length Caudal peduncles length
NN♂ x NN♀ 10 4.21 ± 0.32b 0.61 ± 2.31a 0.61 ± 2.31a 2.56 ± 1.31a 2.61 ± 1.32a 0.61 ± 1.31a 1.80 ± 0.43a 2.30 ± 1.30a 1.20 ± 0.32a
AA♂ x AA♀ 10 5.20 ± 1.31a 0.52 ± 1.31b 0.40 ± 0.61b 2.13 ± 0.22b 2.41 ± 0.13a 0.32 ± 1.34b 1.60 ± 0.31b 2.10 ± 2.30b 1.32 ± 0.36a
NN♂ x AA♀ 10 4.80 ± 0.35b 0.72 ± 1.31a 0.60 ± 0.83a 2.47 ± 1.23a 2.00 ± 0.23b 0.36 ± 0.32b 1.5 ± 0.23b 2.00 ± 1.30b 1.10 ± 0.82b
AA♂ x NN♀ 10 5.30 ± 1.34a 0.51 ± 1.33b 0.50 ± 0.13a 2.12 ± 0.21b 2.20 ± 1.34a 0.52 ± 0.35a 1.8 ± 0.34a 2.41 ± 1.31a 1.00 ± 0.31b

Table 1. Mean and Standard Error of Some morphometric characters of various mating combination for Clarias gariepinus normal and albino Clarias.

The mating combination are as following;

Clarias gariepinus Albino male (♂) x Clarias gariepinus Albino female (♀)

Clarias gariepinus Albino female (♂) x Clarias gariepinus normal male (♀)

Clarias gariepinus normal male (♂) x Clarias gariepinus Albino female (♀)

Normal Clarias gariepinus male (♂) x Normal Clarias gariepinus female (♀).

Keys

NN----NORMAL Clarias Gariepinus

AA----ALBINO Clarias Gariepinus

AA/NN---- ALBINO/NORMAL Clarias gariepinus

NN/AA----NORMAL/ALBINO Clarias gariepinus

The interspecific hybrids however also show some level of positive heterosis in the inheritance of frontal fontanelle length similar to their parents as shown in (Table 1) and also as a reflection of head length as shown in the same table which there were no significant differences in other cephalic traits between the interspecific hybrids and the parental. In the inheritance of the adipose fin length, the dorsal fin, shows no significant difference (P<0.05) in the hybrid of f1. The intermediate morphological traits of the two different colored hybrids suggest that they are product of true fission of the genome of two different colored fishes, except for some of the hybrid especially the reciprocal hybrids of female Albino and normal male (AA♀ x NN♂), male Albino and female normal (AA♂ x NN♀) had brownish color when observed phenotypically as discussed earlier. The findings differed from the finding of Rothbard and Wohlfarth (1993), who reported that inheritance of albinism in grass carp, was as a results of a cross between wild-type heterozygote male and albino female. Among the resulting progeny 52.0% fish were of wild-type color while 48.0% were albino. The crosses of normal pigmented and albino Clarias gariepinus produces normal pigmented heterozygote with white and black patches on the body in agreement of Onyia et al [10]. The findings were relevant with the co-dominant and incomplete dominant cases reported by Murtala and Onyia [10-17]. One allele is not completely dominant over the other. There is a blending with the heterozygous offspring or both alleles contribute to the phenotype. Co dominant is a system in which alleles are from each homozygote parents. Combine in the offspring and the offspring simultaneously demonstrates both parent phenotypes (Table 2).

Mating combination Number sampled Dorsal soft fin rays Pectoral soft fin rays Pelvic soft fin rays Anal soft fin ray Caudal soft fin rays Number of spins in dorsal fin Number of spins in pectoral fin Number of spins in pelvic  fin Number of spins in anal  fin
NN♂ x NN♀ 10 70.50 ± 0.30a 10.50 ± 0.33a 12.50 ± 1.31a 55.00 ± 1.41a 46.50 ± 0.00a 0.0 ± 0.00b 1.00 ± 0.43a 0.00 ± 0.00a 0.00 ± 0.00a
AA♂ x AA♀ 10 70.50 ± 0.32a 10.50 ± 0.33a 12.50 ± 1.31a 55.00 ± 1.41a 46.50 ± 0.00a 0.0 ± 0.00b 1.00 ± 0.43a 0.00 ± 0.00a 0.00 ± 0.00a
NN♂ x AA♀ 10 70.50 ± 0.32a 10.50 ± 0.33a 12.50 ± 1.31a 55.00 ± 1.41a 46.50 ± 0.00a 0.0 ± 0.00b 1.00 ± 0.43a 0.00 ± 0.00a 0.00 ± 0.00a
AA♂ x NN♀ 10 70.50 ± 0.32a 10.50 ± 0.33a 12.50 ± 1.31a 55.00 ± 1.41a 46.50 ± 0.00a 0.0 ± 0.00b 1.00 ± 0.43a 0.00 ± 0.00a 0.00 ± 0.00a

Table 2. Mean and Standard Error of Some meristic characters of various mating combination for Clarias gariepinus normal and albino Clarias.

Conclusion

The finding agreed with the work of Maliszewski who stated that progeny from a wild female and yellow albino male were half wild and half brownish yellow. These reports do not conform to the work of Gomelsky on dihybrid crosses that investigated the colour ratios in progenies obtained after crossing of two-color and tri-color koi. Result show from that study that the white-red color complex and the presence of black patches in koi were inherited independently and the presence of black patches was controlled by the dominant mutation of one gene. Morphological abnormalities observed in some offspring of the mating combination, the skin of the albino cat fish tends to be harder than the normal color C. gariepinus, this was observed during breeding when ovaprim was injected to the females to the parent fish. Therefore, this mating combination seemed to have little to no influence to the external features of the resulting hybrid.

References

  1. Anyanwu PE, Okoro BC, Anyanwu AO, et al. Length-weight relationship, condition factor and sex ratio of    African Mudcatfish (Clarias gariepinus) reared in indoor water recirculation system tanks. J Res Biol Sci. 2007;2:780-3.
  2. Google Scholar

  3. Austin M. Morphometric separation of animal cohorts within mid-Atlantic bluefish, Pomatomus saltatrixusing disciminant function analysis. Fish Bull.1999; 97:411-20.
  4. Bookstein FL. Morphometric tools for landmark data geometry and biology Cambridge. Cambridge University Press. 1991.
  5. Indexed at, Google Scholar

  6. Bronte CR, Fleischer GW, Maistrenko SG. Stock structure of Lake Baikal omul as determined by whole body morphology. J Fish Biol. 1999;54:787-98.
  7. Indexed at, Google Scholar, Cross Ref

  8. Buj I, Podnar M, Mrakovcic M, et al. Morphological and genetic diversity of Sabanejewia Balcaniain Crotia. Folia zool. 2008;57(1-2):100-10.
  9. Google Scholar

  10. Carpenter KE, Sommer HJ, Marcus LF. Converting truss inter landmark distances to Cartesian Coordinates. Advances in morphometrics. ATO Asi Series A. Life Sciences New York Plenum Publ. 1996;284:103-11.
  11. Indexed at, Google ScholarCross Ref

  12. Hubbs CL, Lagler KF. Fishes of the Great Lakes region. Univ Mich Press Ann Arbor Mich. 1958;213.
  13. Gomelsky TA, Murny YM, Mony YJ. Japanese Ornamental Koi Carp: Origin, Variation and Genetics in Biology and Ecology of Carp. 1996;27-53.
  14. Indexed at, Google Scholar

  15. Maliszewsku. Wide reproductive period of a long-distance migratory fish in a subtropical river, Brazil Neotrop Ichthyol. 1982;15(1).
  16. Indexed at, Google Scholar, Cross Ref

  17. Mamuris Z, Apostolidis AP, Panagiotaki P, et al. Morphological variation between red mullet populations in Greece. J Fish Bio.1998;52:107-17.
  18. Indexed at, Google Scholar, Cross Ref

  19. Murtala AG. Diversity and Distribution of Freshwater Fishes in Aceh Water, Northern-Sumatra,Indonesia. J Int  Zool Res. 2009;5(2):62-79.
  20. Indexed at, Google Scholar

  21. Murtala AG. Morphological variation of horse mackerel (Trachurus trachulus) in the Iberian and North African Atlantic; implications for stock identification. J Mar Sci. 2002;57(4):1240-8
  22. Google Scholar, Cross Ref

  23. Naeem M, Salam A. Morphometric study of freshwater bighead Aristichthys nobilis from Pakistan in relation to body size. PJBS. 2005;8(5):759-62.
  24. Google Scholar

  25. Onyia LU, Ochokwu IJ, Akume CP. Growth and survival of normal albino Clarias gariepinus and their reciprocals in hatchery condition. J Nat Sci Res. 2016;4:16.
  26. Onyia LU, Philip B. Jegede OI. Evaluation of reproductive indices and growth of normal albino Clarias gariepinus and their reciprocals in hatchery condition. J Nat Sci Res.2018;8:1.
  27. Google Scholar

  28. Rothband W. A review of ploidy manipulations in aquaculture: The Israeli experience. Isr J Aquac Bamidgeh.1993;58(4).
  29. Google Scholar

  30. Wootton RJ. Ecology of Teleost Fishes. First Edition. Chapman and Hall, London, UK. 1990;13:9780412317200,404.
  31. Google Scholar

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