Short Communication - Journal of RNA and Genomics (2023) Volume 19, Issue 4
Nucleotide modifications and their role in gene regulation.
1Department of Microbiology and Molecular Genetics, Hebrew University of Jerusalem, Jerusalem, Israel
- Corresponding Author:
- Kranzusch Abdullah
Department of Microbiology and Molecular Genetics,
Hebrew University of Jerusalem,
Received: 28-Aug-2023, Manuscript No. RNAI-23-116975; Editor assigned: 30-Aug-2023, Pre QC No. RNAI-23-116975 (PQ); Reviewed: 13-Sep-2023, QC No. RNAI-23-116975; Revised: 21-Sep-2023, Manuscript No. RNAI-23-116975 (R); Published: 29-Sep-2023, DOI:10.35841/2591-7781.19.1000160.
Citation: Abdullah K. Nucleotide modifications and their role in gene regulation. J RNA Genomics 2023;19(4):1-2.
Nucleotides, often referred to as the fundamental building blocks of life, are the essential components that make up the genetic information found in all living organisms. These molecular units play a central role in encoding and transmitting genetic instructions, governing the development, structure, and function of all life forms . Nucleotides serve as the foundation of genetic information storage. Mutations, which are changes in the sequence of nucleotides in DNA, contribute to genetic diversity among individuals and species. Genetic variation is a crucial driver of evolution, enabling organisms to adapt to changing environments . The sequence of nucleotides in DNA carries the genetic code that encodes instructions for protein synthesis and other cellular processes. Through the unique arrangement of these nitrogenous bases, nature encodes the diversity of life and the intricate machinery that governs it. Nucleotides are not solitary entities; they form polynucleotides. In the case of DNA, two complementary polynucleotide strands twist around each other to create the iconic double helix . In RNA, typically, a single polynucleotide strand is found some nucleotides, like Adenosine Tri Phosphate (ATP), serve as carriers of chemical energy within cells. ATP powers many cellular processes, from muscle contraction to active transport across cell membranes. Nucleotides play an integral role in gene expression, including transcription and translation, where genetic information is copied and translated into functional proteins . Nucleotide modifications introduce chemical changes to DNA and RNA, influencing their function. These modifications extend beyond the traditional genetic code, shaping an epigenetic landscape that governs how genes are turned on or off. Key to this landscape are modifications like methylation, acetylation, and phosphorylation. Methylation, involving the addition of methyl groups to cytosine nucleotides, serves as a prominent nucleotide modification in DNA . It silences genes and plays a critical role in the regulation of gene expression. DNA methylation patterns are inheritable and can be influenced by environmental factors, making them a dynamic player in epigenetic inheritance and adaptation . Nucleotide modifications extend to histone proteins, the "spools" around which DNA is wound. Acetylation, methylation, and phosphorylation of histones alter chromatin structure, influencing the accessibility of genes for transcription [7,8]. These marks orchestrate a symphony of gene expression, ensuring that genes are activated or silenced as needed. Nucleotide modifications are not confined to DNA alone . RNA, the messenger that carries genetic information from DNA to protein, can also undergo chemical changes. These RNA modifications, such as m6A (N6-methyladenosine), guide mRNA processing, stability, and translation, thereby impactingprotein synthesis. The significance of nucleotide modifications extends to epigenetic inheritance. Certain epigenetic marks can be passed from one generation to the next, potentially influencing offspring's traits and health . This heritable epigenetic information underscores the importance of understanding nucleotide modifications in the broader context of genetics and biology.
Nucleotides are the cornerstone of genetics and molecular biology. Understanding their structure and function is vital to unraveling the mysteries of life, from the diversity of species to the intricacies of cellular processes. Nucleotide modifications add a layer of fine-tuning to gene regulation. They allow cells to precisely control which genes are expressed and when, ensuring that the appropriate genes are turned on or off to meet the specific needs of the cell, organism, or environmental conditions. Understanding these modifications is critical for unraveling the complexities of development, disease, and the delicate interplay between genetics and epigenetics.
- North RA, Barnard EA. Nucleotide receptors. Curr Opin Neurobiol 1997; 7(3):346-57.
- Sanger F. Determination of nucleotide sequences in DNA. Science 1981; 214(4526):1205-10.
- Cochrane G, Karsch-Mizrachi I, Takagi T. Sequence database collaboration in the international nucleotide sequence database collaboration. Nucleic Acids Res 2016; 44(D1):D48-50.
- Gray IC, Campbell DA, Spurr NK. Single nucleotide polymorphisms as tools in human genetics. Hum Mol Genet 2000; 9(16):2403-8.
- Hoernes TP, Clementi N, Faserl K, et al. Nucleotide modifications within bacterial messenger RNAs regulate their translation and are able to rewire the genetic code. Nucleic Acids Res. 2016; 44(2):852-62.
- Baudin-Baillieu A, Fabret C, Liang XH, et al. Nucleotide modifications in three functionally important regions of the Saccharomyces cerevisiae ribosome affect translation accuracy. Nucleic Acids Res 2009; 37(22):7665-77.
- Helm M. Post-transcriptional nucleotide modification and alternative folding of RNA. Nucleic Acids Res. 2006; 34(2):721-33.
- Malik I, Kelley CP, Wang ET, et al. Molecular mechanisms underlying nucleotide repeat expansion disorders. Nat Rev Mol Cell Biol 2021; 22(9):589-607.
- Stoesser G, Baker W, van den Broek A, et al. The EMBL nucleotide sequence database. Nucleic Acids Res 2002; 30(1):21-6.
- Rafalski A. Applications of single nucleotide polymorphisms in crop genetics. Curr Opin Plant Biol 2002; 5(2):94-100.