Journal of Pharmaceutical Chemistry & Chemical Science

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Opinion Article - Journal of Pharmaceutical Chemistry & Chemical Science (2023) Volume 7, Issue 3

Novel Stationary Phases and Column Technologies in Liquid Chromatography: Enhancing Analytical Performance

Juan Alvarez *

Department of Physical and Analytical Chemistry, University of Jaen, Jaen, Spain

*Corresponding Author:
Juan Alvarez
Department of Physical and Analytical Chemistry,
University of Jaen,
Jaen, Spain

Received:01-Jun-2023, Manuscript No. AAPCCS-23-101863; Editor assigned: 05-Jun-2023, PreQC No. AAPCCS-23-101863(PQ); Reviewed: 19-Jun-2023, QC No. AAPCCS-23-101863; Revised: 23-Jun-2023, Manuscript No. AAPCCS-23-101863; Published: 30-Jun-2023, DOI:10.35841/aapccs-7.3.155

Citation: Alvarez J. Novel stationary phases and column technologies in liquid chromatography: Enhancing analytical Performance. J Pharm Chem Chem Sci 2023;7(3):155




Liquid chromatography (LC) is a widely used separation technique in analytical chemistry, providing efficient and accurate separation of complex mixtures. The performance of liquid chromatography heavily relies on the stationary phase and column technology employed. Over the years, researchers and manufacturers have made significant advancements in developing novel stationary phases and column technologies to enhance the analytical performance of liquid chromatography. These innovations have revolutionized the field, enabling higher resolution, improved selectivity, enhanced sensitivity, and faster separations. In this article, we will explore the key developments in stationary phases and column technologies and their impact on liquid chromatography [1].

Monolithic columns have gained prominence due to their unique structure and advantageous properties. Unlike traditional particle-packed columns, monolithic columns consist of a single continuous porous structure, eliminating interparticle voids. This design allows for faster mass transfer, reduced band broadening, and higher column efficiency. Monolithic columns exhibit lower backpressure, enabling the use of higher flow rates and shorter analysis times without compromising separation quality. These columns are particularly useful for the analysis of large biomolecules, such as proteins and nucleic acids, and have found applications in pharmaceutical, biotechnological, and environmental analysis [2].

Core-shell columns represent another significant advancement in liquid chromatography. These columns consist of a solid core surrounded by a thin layer of porous silica shell. The core-shell design combines the high surface area of porous particles with reduced mass transfer limitations, resulting in improved separation efficiency. Core-shell columns offer enhanced resolution and sensitivity while maintaining reasonable backpressure. With these columns, chromatographers can achieve faster separations without sacrificing separation quality, making them highly valuable in high-throughput analysis and rapid method development [3].

The development of novel hybrid and selective stationary phases has expanded the possibilities of liquid chromatography. Hybrid phases combine multiple functionalities, such as reversed-phase and ion-exchange or reversed-phase and affinity, within a single stationary phase. These versatile phases provide selectivity for a broader range of analytes and enable simultaneous separation of compounds with different properties. Selective stationary phases designed for specific analytes or compound classes have also emerged, offering improved separation selectivity and efficiency. Examples include phases optimized for polar compounds, chiral separations, and hydrophilic interaction chromatography (HILIC) [4].

Porous polymer-based columns have gained attention as an alternative to traditional silica-based columns. These columns utilize porous polymers as the stationary phase, offering unique selectivity, stability, and solvent compatibility. Porous polymer-based columns are particularly advantageous for the separation of hydrophilic compounds and for the analysis of complex samples, such as biological matrices. They are less prone to interactions with analytes and exhibit improved column lifetime, making them an excellent choice for challenging separations [5].


The continuous advancements in stationary phases and column technologies have greatly enhanced the analytical performance of liquid chromatography. The development of monolithic columns, core-shell columns, hybrid and selective stationary phases, and porous polymer-based columns has opened up new possibilities for achieving higher resolution, improved selectivity, enhanced sensitivity, and faster separations. These innovations have revolutionized liquid chromatography, enabling researchers to tackle complex analytical challenges in various fields, including pharmaceuticals, environmental analysis, food safety, and more. As technology continues to evolve, we can anticipate even more exciting developments in liquid chromatography, further enhancing its capabilities and expanding its applications in the future



  1. Wong SH. Novel strategies for clinical drug analysis with new column technology in liquid chromatography. J Pharm Biomed Anal. 1990;8(2):185-93.

    Indexed at, Google Scholar, Cross Ref

  2. García-Gómez D, Rodríguez-Gonzalo E, Carabias-Martínez R. Stationary phases for separation of nucleosides and nucleotides by hydrophilic interaction liquid chromatography. TrAC. 2013;47:111-28.

    Indexed at, Google Scholar, Cross Ref

  3. De Vos J, Stoll D, Buckenmaier S, et al. Advances in ultra?high?pressure and multi?dimensional liquid chromatography instrumentation and workflows. Anal Sci. 2021;2(3-4):171-92.

    Indexed at, Google Scholar, Cross Ref

  4. Wang L, Wei W, Xia Z, et al. Recent advances in materials for stationary phases of mixed-mode high-performance liquid chromatography. TrAC. 2016;80:495-506.

    Indexed at, Google Scholar, Cross Ref

  5. Nazario CE, Silva MR, Franco MS, et al. Evolution in miniaturized column liquid chromatography instrumentation and applications: An overview. J Chromatogr. A. 2015;1421:18-37.

    Indexed at, Google Scholar, Cross Ref

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