Current Pediatric Research

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Research Article - Current Pediatric Research (2021) Volume 25, Issue 5

Effect of iron and zinc supplementation on linear growth of stunted Egyptian children under 5 years of age.

Michael F Fares*, Hanna Aboulghar, Sarah S ElTatawy, Miriam M Aziz

Department of Pediatrics, Cairo University, Cairo, Egypt

Corresponding Author:
Michael F Fares
Department of Pediatrics
Cairo University
Cairo
Egypt
Tel: 201006828040
E-mail: [email protected]

Accepted date: May 6, 2021

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Abstract

Background: Linear growth is the best indicator of children’s well-being and provides an accurate marker of inequalities in human development.

Aim: Study and compare the effect of micronutrients supplementation (Iron and zinc) on linear growth of infants and children less than 5 years of age suffering from stunted growth.

Patients and Methods: A randomized study included 60 children less than 5 years of age came to the department of clinical nutrition in a university hospital for nutritional assessment. Cases randomly divided equally into two groups. Group A received iron supplementation only and group B received supplementation with iron and zinc. Follow up of length/height was done after 6 months of supplementation.

Outcome Measures: Length/height for age and sex (birth to 5 years Z-scores, WHO, 2003).

Results: On comparing the length/ height of the two groups before (p=0.472) and after supplementation (p=0.923) respectively, no statistically significant differences were found between supplementation with iron only and with iron plus zinc. Yet, upon calculation the rate of change in linear growth after 6 months, a significant difference (p=0.002) was evident on comparing group B who received zinc plus iron (mean=13.6 cm increase in height) vs. Group A who only received iron (mean=10.8 cm increase in height).

Conclusion: Zinc supplementation in addition to iron led to significant acceleration of linear growth of stunted children compared to supplementation with iron alone.

Keywords

Stunting, Supplementation, Iron, Zinc, WHO Z-score.

Introduction

Improper nutrition in the first 1,000 days of a human’s life can lead to stunted growth, which is irreversible and accompanied by impaired cognitive ability and reduced school achievement [1]. About 50 percent of all children suffering from stunting lived in Asia and more than one-third in Africa [2].

Micronutrients play a major role in learning, cognitive functions, immune responses and cellular signaling. A study done in USA included children up to 18 years and found evidence that zinc supplementation had medium effect in improving height among children, especially who were young age and stunted before supplementation [3].

Iron has vital functions like acting as a co-factor for enzymatic reactions and carrying of oxygen to the tissues from the lungs. Iron deficiency worsen oxygen dependent cellular energy metabolism consequently, leads to defective linear growth and impaired cognition [4].

Patients and Methods

A randomized study was performed on stunted Egyptian children less than 5 years of age attended for nutritional assessment. An informed consent was obtained from the parents of all enrolled infants and children.

The study included 60 children less than 5 years of age suffering from stunted growth in the period from 1/5/2017–1/5/2018. Stunting was identified by assessing a child’s length or height (recumbent length for children less than 2 years old using a ruler and standing height for children age 2 years or older using stadiometer) and put the measurements on WHO Z-scores.

Exclusion criteria

• Children over 5 years of age.

• Ex-Premature infants.

• Children with physical growth disabilities, mental retardation or chronic illness such as sickle cell disease, cystic fibrosis, or severe protein-energy malnutrition.

Sample size

Sample size was calculated based on the previous work by Mozaffari-Khosravi et al. and Petry et al. the expected difference of height for age between the two groups was 1 ± 1.2 [5,6]. Using power 80% and 5% significance level; 24 participants in each group were required. This number was increased to 30 to compensate for possible losses during follow up. Sample size calculation was achieved using. PS: Power and Sample Size Calculation software Version3.1.2 (Vanderbilt University, Nashville, Tennessee, USA).

Cases evaluation

All cases were collected from the clinical nutrition department in a university. Following admission and enrollment, full history taking including age, gender, and prenatal, dietary and developmental history were taken together with a thorough clinical examination. MyPlate and 24-hour dietary recall assessment were used for proper assessment of food diversity and quantity Initial Anthropometric measurements (using WHO Z scores) to assess height or length/age for boys and girls [7-10]. Stunting uses a Z-score cut-off point of <-2 SD to classify low height-for-age as moderate and <-3 SD to classify severe.

Iron and zinc supplementation

Recommendations for zinc intake was 10 mg/day in children up to 5 years of age which make severe zinc deficiency much less common in developed countries [11]. Iron was given in the dose of 10-12.5 mg elemental iron for children up to 2 years of age. Children aged 2-5 years received 30 mg elemental iron according to WHO guidelines [12].

Follow up data

Follow up sessions for all the included cases every 2 weeks to ensure that they were compliant with the supplements. Assessment of length/height was done after 6 months using WHO Z-scores under 5 years of age. Compared to percentiles, Z-scores have a number of advantages: they are calculated based on the distribution of the reference population and comparable across ages, sexes, and anthropometric measures [13].

Statistical data

Data management and analysis were performed using SPSSc Statistics version 24 (IBMc Corp., Armonk, NY, USA). Comparisons between groups with respect to numeric variables were done using the Student’s t-test for normally distributed variables and the Mann-Whitney nonparametric test for the non-normally distributed variables. Comparisons between categorical variables were done by the chi-square test or fisher's exact for small sample size. All p-values are two-sided. P<0.05 were considered significant.

Results

Out of 60 enrolled children, 43% of them lived in rural areas and 57% were from urban areas. Prenatal history revealed that 40% of the mothers in group A suffered from anemia during pregnancy compared to 30% in group B. Percentage of infants who were received exclusive breast fed in group A was 76.7% vs. 60.0% of group B. Over 23% of infants in group A were formula fedvs. 40% of group B as shown in (Table 1).

  Group A Group B
Count n=30 % Count n=30 %

Residency

Rural

16 53.30% 10 33.30%

Urban

14 46.70% 20 66.70%

Non educated parents

18 60% 17 56.60%

Prenatal history mothers suffered from anemia

12 40% 9 30%

Exclusive breast feeding

23 76.70% 18 60.00%

Formula feeding

7 23.30% 12 40.00%

GIT infection

7 23.30% 9 30.00%

Regarding diversity of the dietary elements, (Table 2) showed that 78.6% of the weaned children in group A had improper diversity in their meals and 71.4% didn’t eat proper quantity of food they should take. In group B 86.2% of the weaned children had improper diversity in their meals and 72.4% didn’t eat proper quantity of food they should take.

Diversity and quantity of food Group A Group B
Count n=28 % Count n=29 %
Diversity Proper 6 21.40% 4 13.80%
Improper 22 78.60% 25 86.20%
Quantity Proper 8 28.60% 8 27.60%
Improper 20 71.40% 21 72.40%

Proteins was the major deficient element inthe food of 46.7% of group A vs. 56.7% of group B as illustrated in (Figure 1). This was followed by lack of carbohydrates and vegetables (each 20%) then Fruits and Dairy products (each 13.3%) in the diet of group A. The other food elements deficient in group B were carbohydrates and fruits (each 26.7%) then vegetables and dairy products (each 13.3%).

Figure 1: Deficient elements in the meals received by the studied groups.

Assessment of developmental milestones showed that 46.7% had delayed cognitive skills in group A compared to 36.7% in group B. Delayed motor milestones were found in 33.3% of group A compared to 23.3% in group B as shown in (Table 3).

Delayed developmental milestones Group A Group B
Count n=30 % Count n=30 %
Motor development 10 33.30% 7 23.30%
Cognitive skills 14 46.70% 11 36.70%
Speech 5 16.70% 6 20.00%
Social development 10 33.30% 6 20.00%

Correlation of the gender to height/length in the initial visit revealed no statistically significant difference (Table 4). Median age of included cases was 13 months and there was no bias regarding age between the two groups (p=0.6). Also, on comparing length/height of both studied groups together there was no statistically significant difference between them in the first visit so no bias regarding to the initial length of children (p=0.472) as seen in (Table 5). Upon calculation the rate of change in length/height, a significant difference was found between the two groups on follow up after 6 months (p=0.002).

Groups Gender P value
Male Female
N Mean Standard deviation Median Minimum Maximum N Mean Standard deviation Median Minimum Maximum
A 19 71 8.4 68.5 59 90 11 70 9.2 67 59 90 0.641
B 8 70 6.5 70 61 81 22 69 8.8 66 59 97.5 0.344
Length/Height Group A Group B P value
Mean Standard deviation Median Minimum Maximum Mean Standard deviation Median Minimum Maximum
Age months 17.1 11.6 13.5 6 51 15.2 10 13 5 56 0.602
On the first visit 70.6 8.6 68 59 90 69 8.2 67 59 97.5 0.472
Rate of Change (in cms) 10.8 2.8 11.1 6 15 13.6 3.7 13.6 5.6 19.4 0.002*

After 6 months of micronutrient supplementation, evaluation of each group separately showed that there was a statistically significant increase in length/height of each group (P-value<0.001) (Table 6). Meanwhile, (Figure 2) demonstrated that 60.0% of cases in group A became <-2 on WHO Z-score vs. 26.7% in group B and 40.0% in group A became =-2 compared to 73.3% in group B. Comparing of both groups together showed that the length/height of group B which received supplementation with iron plus zinc was significantly increased compared to group A which received supplementation with iron only (p-value=0.009).

Length/height Group A Group B
Mean Standard deviation Median Minimum Maximum Mean Standard deviation Median Minimum Maximum
On the first visit 70.6 8.6 68 59 90 69 8.2 67 59 97.5
After 6 months 78 7.5 75 68 95 78.2 7.1 76 70 103
P-value <0.001* <0.001*

Figure 2: Height/length before and after micronutrients supplementation on who z-score.

Discussion

The aim of this study was to systematically evaluate the effect of iron and zinc supplementation on linear growth of infants and children below 5 years of age. The study was a randomized study including 60 children less than 5 years of age. They were randomly divided into two groups. Group A received iron only and group B received iron and zinc. Follow up of length/height was done after 6 months of supplementation.

As for place of residence, Shortage of economic resources means that food insecurity is more common among rural inhabitants [14]. Teenage pregnancy and high parity were prevalent in rural areas which cause nutrients depletion from the mothers because of physiological immaturity and the stresses of multiple pregnancies with short intervals in between [15].

About 60% of parents of the children included in our study were not educated. A study done by Vollmer et al. [16] in 2017 on childhood malnutrition in low-income countries reported a higher percentage of stunted children have non educated parents.

Many of children in our study received treatment for parasitic infestations. Many studies showed that parasitic infestation is common and has adverse outcomes on child growth and development. There is a increasing appreciation of the burden of helminths in pre-school children in whom infection may have the strong impact on child health [17].

About 50% of mothers in our study suffered from anemia during pregnancy. An intervention study examined the effect of nutrition on improving maternal and infant health in developing communities during pregnancy reported that African countries showed higher prevalence of maternal overweight, associated with poor quality of food and micronutrients deficiencies in women suffered from chronic malnutrition [18].

Delay in motor milestones and a cognitive skill among our studied groups was about 30%-40% in patients of each group while delay in speech and social development was about 20%-30%. This came in line with a study done in Nigeria by Jimoh et al. in which reported a prevalence of developmental delay of 35.4% among the study population [19]. Lower figures were reported among 9 to 24 months old American children Rosenberg et al. [20]. The cause for this variance may be related to several factors such as age range, number of developmental varieties assessed, method of assessment, wealth of countries and socioeconomic state.

In our study, 68% of children were Exclusively Breast Fed (EBF) and 32% received formula feeding in addition to breast feeding. A study done in Indonesia found that most of the subjects were breastfed for two years. This study reported that prolonged breast feeding duration was associated with increasing incidence of stunting [21].

About 30% of infants involved in this study were fed on cow’s milk. A study done by Zieglershowed that the feeding of cow’s milk has adverse effects on iron nutrition in infants [22]. Mostly due to low iron level in cow’s milk, occult intestinal blood loss and also high amounts of calcium and casein present in cow’s milk inhibit the absorption of dietary non heme iron.

Lack of diversity in the food was noticed in about 80% of children integrated in this study and about 70% of them didn’t receive quantity of food appropriate for their age. This was consistent with the results of a study conducted in Nepal [23]. This study found that a more diverse diet is beneficial for child growth. Another study conducted in Ethiopia mentioned that meals number and food diversity the child ate per day significantly affect the prevalence of stunting [24].

Our study showed that the element most deficient in the diet of stunted children was protein. A study done in rural southern Malawi by Semba et al. found that stunted children had lower serum concentrations of all essential amino acids compared with normal children [25]. In addition, stunted children had significantly lower serum concentrations of conditionally essential amino acids (arginine, glycine, and glutamine), non-essential amino acids and six different sphingolipids. If amino acids are not sufficient to synthesize proteins, growth regulatory pathways (mTORC1 and GCN2) will not allow growth to proceed [26].

We didn’t report significant difference in linear growth according to gender in our country. However, in India a study done by Jawaregowda and Angadifound that gender inequalities in food quality and quantity may contribute to malnutrition especially in settings where female off-springs are still considered less important compared to males [27].

According to Height for Age Z-score (HAZ) in our study, 80.0% of group A were placed on <-2 versus 63.3% of group B and 20.0% of group A were placed on <-3 versus 36.7% of group B. The rate of change after 6 months was 10.8 ± 2.8 in group A versus 13.6 ± 3.7 in group B. In a study done in Kenya by Ndiku et al. The percentage of children suffered from severe stunting (HAZ>-3) was 20.5% and this similar to the results of our study [28].

On comparing the length/height of the two groups before supplementation, no statistically significant differences were found between the two groups (p=0.472). After six months supplementation with micronutrients, the length/height of stunted children in each groups respectively was significantly improved (p<0.001).

Upon calculation the rate of change in linear growth after 6 months, a significant difference (p=0.002) was evident on comparing group B who received zinc plus iron (mean=13.6 cm increase in height) versus group A who only received iron (mean=10.8 cm increase in height). This statistically significant difference was also evident by plotting the length/height of the two groups on Z-score, as 73% of group B rose above the -2 value on Z-score compared to only 40% of group A (p=0.009).

These results were in agreement with a meta-analysis done by Imdad et al. which found that zinc supplementation is associated with a net benefit on linear growth. Results from a study by Fahmida et al. were in concordance with our results. This study showed that the benefit of the zinc plus iron supplementations were significantly higher among initially stunted cases [29,30].

The type of zinc supplementation used in our study was zinc sulfate and this is in agreement with most studies. In this study we gave zinc supplementation in a dose 10 mg/day. This came in line with a study by Dijkhuizen et al. which showed that preventive zinc supplementation in a dose of 10 mg/day has the most significant effect on linear length in children <5 years of age [31].

Conclusion

In our study iron supplementation alone without zinc also improved linear growth of stunted children. Our results are in line with a study conducted in Egypt by Ibrahim et al. which showed that iron is crucial for the growth of infants and preschool children in Egypt. In this study, a significant acceleration of subjects’ growth velocities were observed after iron supplementation for 6 months, associated with improvement in their height SDS.

Key Message

Assessment of anthropometric measures is an essential part in examination of infants and children to pick up any case of stunting early.

Dietary iron supplementation is recommended for exclusively breastfed term infants starting at four to six months of age. Exclusive breastfeeding after six months puts infants at risk for iron deficiency.

Regular dietary zinc intake is required because zinc cannot be produced or stored in the body.

Declaration of Competing Interest

All authors have approved the final article and have no conflict of interest to declare.

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

References

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