Short Article - Journal of Clinical and Bioanalytical Chemistry (2021) Volume 5, Issue 3
Mass spectrometry- based proteomics: Past, present and future
Protein purification, characterization and useful analysis play an important role in understanding the biology of the cells. Earlier, sequencing of proteins by Edmann degradation technique has been used for the identification of proteins for a protracted time. However, the complexities related to these long processes necessitated the event of recent technologies that might be users-friendly and need less time. With the event of sentimental ionization techniques in mass spectrum analysis, supermolecule identification, quantification and detection of the post -translational modifications became a routine observe in many laboratories. Detection of false discoveries in supermolecule identification, and discrepancies among completely different quantification strategies junction rectifier to the continual evolution of Mass spectrum analysis strategies. the utilization of various ionization strategies viz, Collision elicited Dissociation (CID), High Energy Collision (HCD), negatron Transfer Dissociation (ETD) and negatron Capture Dissociation (ECD) is advantageous for sequencing of the peptides/proteins. additionally to the current, chemical modification of peptides followed by mass spectrum analysis helps in up the sequencing potency of peptides. A comparative study among completely different quantification strategies, and issues related to each info and First State novo sequencing strategies are going to be mentioned. each top-down and bottom-up approaches are going to be explained with relevant examples. the long run prospects within the technological developments will be highlighted.
Author(s): M. V. Jagannadham