Short Communication - Immunology Case Reports (2022) Volume 0, Issue 0

Investigation of the Effects of B16F10 Derived Exosomes in Induction of Immunosuppressive Response in the Stem cells

One of the main mechanisms of tumor cells' escape from destruction by the immune system is the suppression of antitumor immune responses. Myeloid-derived suppressor cells (MDSCs) are the main immunosuppressive cells present in the tumor microenvironment (TME) that sustain tumor progression. MDSCs are a heterogeneous group of cells including granulocytic MDSC (G-MDSCs) and monocytes MDSC (Mo-MDSCs) which originate from immature myeloid cells. Tumor cell-derived exosomes (TEX) can deliver their cargoes to myeloid cells and convert them into MDSC. Herein, the researchers investigated the effects of exosomes from B16F10 mouse melanoma cells on the development of hematopoietic stem cells (HSC) into MDSC subtypes. Briefly, the exosomes were isolated from the cultured B16F10 cell line. We isolated HSC from the C57BL6 mouse model of melanoma cancer. Differentiation of the HSC into the MDSC was done through the treatment of the HSC with exosomes. To confirm the differentiation of the HSC cells, the researchers performed the expression of markers of MDSC with flow cytometry. In addition, we measured the cytokine secretion in the supernatant with ELISA 

Author(s): Mohammad Reza

Abstract PDF