Immunology Case Reports

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Short Communication - Immunology Case Reports (2021) Volume 4, Issue 1

Imaging in cancer immunology: Phenotyping of multiple immune cell subsets in situ in FFPE tissue sections

immunobiology in recent years as a result of recent
successes in cancer immunotherapies and it is becoming
clear that immune cells play many sometimes conflicting
roles in the tumor microenvironment. However, obtaining
phenotypic information about the various immune cells that
play these roles in and around the tumor has been a
challenge. Existing methods can either deliver phenotypic
information on homogenous samples (e.g., flow cytometry
or PCR) or morphologic information on single
immunomarkers (standard IHC). We present here a
methodology for delivering quantitative per-cell marker
expression and phenotyping, analogous to that obtained
from flow cytometry but from cells imaged in situ in FFPE
tissue sections. This methodology combines the sequential
multi-marker labeling of up to 6 antigens using antibodies
all of the same species in a single section; automated
multispectral imaging (MSI) to remove the typically
problematic FFPE tissue auto fluorescence and correct
cross-talk between fluorescent channels and an automated
image analysis that can quantitate the per-cell marker
expression, determine the cellular phenotype, count these
cells separately in the tumor compartment and in the stroma


Author(s): Maciej P Zerkowski

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